ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Study on the biological characteristics of a new arbovirus: Nam Dinh virus
Received date: 2016-04-15
Online published: 2016-10-20
Supported by
Supported by the Natural Science Foundation of Guangdong Province of China (No. 2014A030313583)
Objective To investigate the immunological characteristics and pathogenicity of China's first isolated mosquito borne viruses Nam Dinh virus (NDiV) on cultured cells and Kunming mouse. Methods The following methods were used:C6/36 cells for observation on cytopathic effect (CPE), plaque assay for determination of NDiV titer, virus inoculation methods for detection of NDiV virulence in 3 days old mice brain. In C6/36 cells, neutralization test was done using neutralizing antibodies from immunized Kunming mice. The BHK21 cells were inoculated with virus neutralizing antibodies of NDiV, one hour exposure followed by culturing for 7-9 d. Daily observation was made and the cytopathic effect was recorded. The TaqMan-MGB Probe Real-time PCR method was used to detect the nucleic acid of NDiV in C6/36 cells. Results C6/36 cells were infected after incubation with NDiV, CPE appeared since fifth days, the virus titer was 9.25×106 PFU/ml, the LD50 was 104.12/ml by Reed-Muench method. After NDiV virus infected mice, strong immunogenicity and higher rate of virus protection were showed in NDiV virus antiserum group, TCID50 was less than 10-1 while the control group TCID50 for 10-3.5. The neutralization index greater than 1 000. The virus nucleic acid detection results indicated positive 3 d after inoculation, virus nucleic acid showed significantly positive with growth of virus post inoculation upon virus nucleic acid increase. Conclusion The NDiV has certain pathogenicity and antigenicity against the exposed C6/36 cells and Kunming mouse.
Key words： Nam Dinh virus; Biological characteristics; Toxicity
WANG De-quan, LIU Qu, LIANG Ke-feng, ZHOU Jian-ming, ZHOU Jun-li, LIU Xiao-na . Study on the biological characteristics of a new arbovirus: Nam Dinh virus[J]. Chinese Journal of Vector Biology and Control, 2016 , 27(5) : 443 -446 . DOI: 10.11853/j.issn.1003.8280.2016.05.005
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