ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Identification of 8 female Sarcopha flies based on mitochondrial DNA and signum morphological characteristics
Received date: 2016-06-16
Online published: 2016-10-20
Supported by
Supported by the National Science and Technology Infrastructure Program (No. 2012BAK11B05), Administration of Quality Supervision, Inspection and Quarantine Support Program of China (No. 2015IK067, 2015IK069), and Science and Technology Project of Guangdong Province (No. 2015A050502009)
Objective To establish a method to accurately identify the species of the female Sarcopha. Methods Thirty five mating pairs of eight Sarcopha species and 57 single male individuals of these 8 species were collected from October 25 in 2011 to October 27 in 2013. Their DNA barcodes were sequenced and 11 DNA barcodes sequences of these 8 species were also downloaded from the GenBank. Males were identified according to their genitalia, DNA barcodes differences between the intra-species, inter-sexes and inter-species were compared. Females were identified according to their mating males and the DNA barcodes, female's signum morphological characteristics of these eight species were illustrated and a key based on it was constructed. Results Inter-sexes DNA barcodes differences were 0-1.37% of these 8 species, and were comparable to the intra-species variations, and were lower than the 2% species limitation. The inter-species DNA barcodes differences were 4.56%-8.81%, there are distinct gaps between the intra-species and inter-species. The signum morphological characteristics were species-specific and could be used to identify the females. Conclusion DNA barcoding is a practical tool to identify the female Sarcopha. Species-specific morphological characters of the female could be developed based on the identification result of DNA barcoding, then provides references for the direct morphological identification of the females.
YUE Qiao-yun, QIU De-yi, SHAN Zhen-ju, WANG Xiao-dong, HU Jia, ZHANG Wen-qing . Identification of 8 female Sarcopha flies based on mitochondrial DNA and signum morphological characteristics[J]. Chinese Journal of Vector Biology and Control, 2016 , 27(5) : 436 -442 . DOI: 10.11853/j.issn.1003.8280.2016.05.004
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