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Chines Journal of Vector Biology and Control ›› 2009, Vol. 20 ›› Issue (6): 534-537.
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ZHAO Bo, WANG Ling, WANG He, WANG Wei-Jie, QI Li-Li, LI Ze-Min
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赵博1,王玲2,王贺1,王卫杰1,齐莉莉1,李泽民1
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Abstract:
【Abstract】 Objective Survey the morphological changes of Lucilia sericata larvae at different temperature and screen out the indexes deducing larvae age. Methods The necrophagous fly L. sericata were reared at constant temperature of 20 ℃,24 ℃ and 28 ℃ respectively and were tempted to lay eggs with lean pork. One Sample was taken at 12 h intervals till 50% of the larvae were in pupae stage. Then, the anterior spiracle,posterior spiracle and cephalopharyngeal skeleton were investigated through digital image processing combined with morphologic observation. Results The anterior spiracle,posterior spiracle and cephalopharyngeal skeleton regularly changed with daily larval growth. The three indexes including the sclerotizes area of pharyngeal sclerite, the average optical density of pharyngeal sclerite and the posterior spiracle could be used as the ideal indexes of larvae age. Conclusion The regular changes of the three sections at constant temperature would provide some scientific database for the exact deducing of the postmortem interval.
Key words: Forensic entomology, Postmortem interval, Lucilia sericata, Cephalopharyngeal skeleton, Spiracle
摘要:
【摘要】 目的 观察不同温度下丝光绿蝇幼虫形态学变化筛选日龄推断的指标。方法 在20 ℃、24 ℃和28 ℃下饲养丝光绿蝇,瘦猪肉诱其产卵,每隔12 h取样1次,直至50%以上幼虫化蛹为止,采用形态观察与图像分析相结合的方法对幼虫前气门、后气门、头咽骨进行观察研究。结果 丝光绿蝇幼虫前气门、后气门、头咽骨均随着时间的延长发生规律性变化,幼虫后气门平均光密度、咽骨骨化面积及其平均光密度这3项指标是推断幼虫日龄较理想的指标。结论 在恒温条件下,幼虫前气门、后气门、头咽骨均发生规律性变化,为法医学中尸体死后间隔时间的精确推断提供了一定的理论依据。
关键词: 法医昆虫学, 死后间隔时间, 丝光绿蝇, 头咽骨, 气门
CLC Number:
R384.2
ZHAO Bo, WANG Ling, WANG He, WANG Wei-Jie, QI Li-Li, LI Ze-Min. Morphological changes of Lucilia sericata larvae and its significance in forensic medicine[J]. Chines Journal of Vector Biology and Control, 2009, 20(6): 534-537.
赵博1,王玲2,王贺1,王卫杰1,齐莉莉1,李泽民1. 丝光绿蝇幼虫不同温度下的形态学变化及法医学意义[J]. 中国媒介生物学及控制杂志, 2009, 20(6): 534-537.
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