Chines Journal of Vector Biology and Control ›› 2016, Vol. 27 ›› Issue (2): 141-144.DOI: 10.11853/j.issn.1003.8280.2016.02.012

• Original reports • Previous Articles     Next Articles

The application of tandem repeat in genetic typing Yersinia pestis of Hebei province

WANG Hai-feng, YANG Shun-lin, ZHOU Song, BAI Xue-wei, ZHANG Yi-hui, YANG Xiao-yan, NIU Yan-fen, LIU He-zhi, DU Guo-yi, SHI Xian-ming   

  1. Anti-plague Institute of Hebei Province, Zhangjiakou 075000, Hebei Province, China
  • Received:2015-12-21 Online:2016-04-20 Published:2016-04-20
  • Supported by:

    Supported by the Youth Science and Technology Project of Hebei Provincial Health Department (No. ZL20140119)

可变数目串联重复序列在河北省鼠疫菌株分型中的应用

王海峰, 杨顺林, 周松, 白雪薇, 张懿晖, 杨晓燕, 牛艳芬, 刘合智, 杜国义, 史献明   

  1. 河北省鼠疫防治所检验科, 河北张家口 075000
  • 通讯作者: 史献明,Email:wolfshi9974@sina.com
  • 作者简介:王海峰,女,硕士,主管检验师,从事鼠疫检验研究,Email:whf584521@163.com
  • 基金资助:

    河北省卫生厅青年科技课题(ZL20140119)

Abstract:

Objective To study the genotype of Yersinia pestis in Hebei plague foci by variable number of tandem repeat (VNTR). Methods Primers were designed according to the confirmed 14+12 VNTR, to genotype the 116 Y. pestis DNA of Hebei province. Results All of the strains showed one genetype, but they were different from CO92 and EV. Conclusion There is only one genetype of plague, indicating a genetic stability in Y. pestis in Hebei province.

Key words: Yersinia pestis, Variable number of tandem repeat, Genotyping

摘要:

目的 利用可变数目串联重复序列(VNTR)对河北省鼠疫疫源地菌株进行基因型别研究。方法 根据14+12对VNTR设计引物,对116株鼠疫菌进行分析。结果 116株鼠疫菌针对每对VNTR均有不同的重复数目,但是同一对VNTR对所有菌株的VNTR重复数目相同。与CO92和EV对比,河北省鼠疫疫源地菌株显示出不同的扩增结果。结论 河北省鼠疫疫源地鼠疫菌只有1种不同于其他疫源地的基因型别,为疫情的追踪溯源和鼠疫菌突变监测提供了依据。

关键词: 鼠疫菌, 可变数目串联重复序列, 基因分型

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