Investigation of infection with Bartonella in domestic and feral cats in Beijing, China

doi:10.11853/j.issn.1003.4692.2015.01.005

Chines Journal of Vector Biology and Control ›› 2015, Vol. 26 ›› Issue (1): 19-22.DOI: 10.11853/j.issn.1003.4692.2015.01.005

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Investigation of infection with Bartonella in domestic and feral cats in Beijing, China

HUANG Ru-ting^1,2, SONG Xiu-ping¹, YANG Xiu-huan³, LI Dong-mei¹, ZHAO Fan¹, SUN Ji-min¹, LIU Qi-yong¹

1. Department of Vector Biology and Control, State Key Laboratory for Infectious Diseases Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
2. Fengtai Center for Disease Control and Prevention, Beijing 100071, China;
3. Beijing Veterinary Station

Received:2014-09-30 Online:2015-02-20 Published:2015-02-20
Supported by:
Supported by the Major National Science and Technology Subject(No. 2008ZX10004-010)

北京市宠物猫和流浪猫巴尔通体感染状况调查

黄儒婷^1,2, 宋秀平¹, 杨秀环³, 栗冬梅¹, 赵帆¹, 孙继民¹, 刘起勇¹

1. 中国疾病预防控制中心传染病预防控制所, 传染病预防控制国家重点实验室, 北京102206;
2. 北京市丰台区疾病预防控制中心, 北京100071;
3. 北京市畜牧兽医总站

通讯作者: 刘起勇,Email: liuqiyong@icdc.cn
作者简介:黄儒婷,女,硕士,医师,主要从事病媒生物的防制研究,Email: rutingcc@163.com
基金资助:
国家科技重大专项课题(2008ZX10004-010)

Abstract

Abstract:

Objective To investigate the status of infection with Bartonella in domestic and feral cats in Beijing, China. Methods EDTA-anticoagulated blood and serum samples were collected from cats, and the relevant epidemiological information was recorded. The anticoagulated blood was diluted 1:4 in sterilized tryptic soy broth and a 100 μl aliquot was inoculated onto the brain-heart infusion agar supplemented with 5% defibrinated sheep blood followed by culturing in a 5% CO₂ incubator at 37 ℃ for 45 days. Bartonella-like isolates from the culture were examined by PCR analysis using the primers for gltA, ftsZ, and ribC. The PCR products were sequenced, and homologous comparison and phylogenetic analysis were performed to identify the species of Bartonella. Indirect immunofluorescence assay was carried out to measure the titer of anti-Bartonella henselae IIFT (IgG) in the serum samples. Data obtained from the experiments and the epidemiological information collected in the field were analyzed by the SPSS 13.0 software. Results The positive rate for Bartonella culture isolates was 13.8% in the cats tested in Beijing, and all the 22 strains obtained were B. henselae. The seropositivity rate for anti-B. henselae IgG was 39.4%. The feral cats, flea-infested cats, and kittens had significantly higher positive rates for Bartonella isolates (30.4%, 36.6%, and 27.9%, respectively). The seropositivity rate for anti-B. henselae IgG in flea-infested cats (61.0%) was also significantly higher than that in uninfested cats (31.9%). Conclusion Bartonella infection is common in both domestic and feral cats in Beijing, and the species is identified to be human pathogen B. henselae. Bartonella infection in humans could be avoided by flea control and preventing flea infestation in domestic cats, and strengthening the control of feral cats.

Key words: Bartonella, Domestic cat, Feral cat, Cat scratch disease

摘要：

目的调查北京市宠物猫和流浪猫巴尔通体感染状况。方法采集猫的抗凝血和血清并收集相关流行病学信息。将抗凝血用灭菌胰酶大豆肉汤按1:4稀释后,取100 μl接种于含5%去纤维羊血的脑心浸液培养基上,置于37 ℃、含5%CO₂培养箱中分离培养至45 d。选择gltA、ftsZ、ribC 引物对分离到的疑似菌落进行PCR并测序,所测核酸序列进行同源性比较及系统发育分析,确定巴尔通体种。利用间接免疫荧光法检测血清样本汉赛巴尔通体抗体水平。利用SPSS 13.0软件分析实验室数据与现场采集的流行病学数据。结果北京市猫的巴尔通体血培养分离率为13.8%,获得的22 株分离株全部为汉赛巴尔通体。血清抗体阳性率为39.4%。流浪猫(30.4%)、染蚤猫(36.6%)、幼猫(27.9%)的血培养阳性率较高,差异有统计学意义。染蚤猫的血清抗体阳性率(61.0%)也显著高于未染蚤猫(31.9%)。结论北京市宠物猫和流浪猫中巴尔通体感染率较高,且均为对人致病的汉赛巴尔通体,需做好宠物猫的防蚤除蚤、流浪猫的管理来预防人类巴尔通体感染。

关键词: 巴尔通体, 宠物猫, 流浪猫, 猫抓病

CLC Number:

Q939.93

HUANG Ru-ting, SONG Xiu-ping, YANG Xiu-huan, LI Dong-mei, ZHAO Fan, SUN Ji-min, LIU Qi-yong. Investigation of infection with Bartonella in domestic and feral cats in Beijing, China[J]. Chines Journal of Vector Biology and Control, 2015, 26(1): 19-22.

黄儒婷, 宋秀平, 杨秀环, 栗冬梅, 赵帆, 孙继民, 刘起勇. 北京市宠物猫和流浪猫巴尔通体感染状况调查[J]. 中国媒介生物学及控制杂志, 2015, 26(1): 19-22.

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Investigation of infection with Bartonella in domestic and feral cats in Beijing, China

北京市宠物猫和流浪猫巴尔通体感染状况调查

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