Abstract:

Objective To describe the symptoms of Lucilia sericata infected with Pandora dipterigena and the morphology of P. dipterigena, providing the basis for blowfly control. Methods The morphology of P. dipterigena was observed using aceto-orcein and cotton blue staining. The diameter of conidia, nuclei and conidiophores were measured and photographed. Results The typical adults of L. sericata infected with P. dipterigena usually attached to the stems of Ficus virens, wings spread laterally, with a dense and gray conidiophore layer covering the whole body. Around the body was intensively protruding pseudocystidia of different lengths. The primary conidia was bitunicate with a single nucleus, varying in form from elongated ellipse to subfusiform, (24.8±1.8)μm×(10.8±0.9)μm [(22.1-28.6 μm)×(9.1-14.3 μm)] in diameter with L/D of 2.3±0.2(1.89-2.86). The secondary conidia was similar to the primaries in the appearance, (17.3±1.4)μm×(11.5±1.0)μm [(15.6-19.5 μm)×(9.1-13.0 μm)], L/D=1.5±0.1(1.3-1.7). The pseudocystidia was abundant and monomitic, with a basal diameter of (19.7±1.3)μm (16.2-22.9 μm) and (5.4±1.2)μm (4.0-6.1 μm) to the ends. The rhizoids were monomitic with the diameter of (19.5±0.9)μm (16.6-21.4 μm), with a disc-like holdfast. No resting spores were observed. Conclusion The fungus P. dipterigena may be used in the control of L. sericata.

Key words: Lucilia sericata, Pandora dipterigena, Entomophthorales, Entomopathogenic fungi, Guangdong province

摘要：

目的 描述丝光绿蝇感染双翅目虫疠霉的症状及其虫疠霉的形态特征，为控制丝光绿蝇提供科学依据。方法 醋酸地衣红和棉蓝染色法观察双翅目虫疠霉的形态，分别显微测量分生孢子、细胞核和分生孢子梗直径，并摄影。 结果 感染双翅目虫疠霉的丝光绿蝇成虫附着于大叶榕的树干上，双翅向侧上方张开，浓密的灰白色虫霉子实层几乎覆盖整个虫体，四周可见很多密集的、长短不一的假囊状体伸出。双翅目虫疠霉的初生分生孢子长椭圆形至近纺锤形，(24.8±1.8)μm×(10.8±0.9)μm〔(22.1～28.6 μm)×(9.1～14.3 μm)〕，长径比(L/D)为2.3±0.2 (1.89～2.86)， 双囊壁， 单核。次生分生孢子形似初生分生孢子(17.3±1.4) μm×(11.5±1.0)μm〔(15.6～19.5 μm)×(9.1～13.0 μm)〕，L/D为1.5±0.1(1.3～1.7)。假囊状体多， 单菌丝状，基部φ(19.7±1.3)μm (16.2～22.9 μm)，端部φ(5.4±1.2)μm (4.0～6.1 μm)。假根单菌丝状，φ(19.5±0.9)μm (16.6～21.4 μm)，端部分化为盘状固着器。休眠孢子未见。结论 双翅目虫疠霉可能对丝光绿蝇有控制潜力。

关键词: 丝光绿蝇, 双翅目虫疠霉, 虫霉目, 昆虫病原真菌, 广东省