Abstract: Objective:To determine the frequency and characteristics of reassortment among viruses causing hemorrhagic fever with renal syndrome(HFRS).Methods:Mixed infections were initiated in tissue culture by using Hantaan virus strains(76-118) and Seoul virus strains(R22).Potential reassortant virus plaques were screened by multiplex RT-PCR,using primers specific for individual genome segments of each strain.Results:Most of the progeny virus plaques(70.83% of 24) had the parental genotype of 76-118 strain or R22 strain.1 of 24 plaques had mixed genotypes that yielded RT-PCR bands for the same segment of both parental strains.Reassortant viruses were detected in 16.67% of 24 progeny plaques tested,involving the M and L segments.In addition,approximately 4.17% of the progeny virus plaques appeared to contain M segments originating from both parental virus strains,i.e.they were diploid.Conclusion:Genetic reassortment can occur between Hantaan virus strains and Seoul virus strains.

摘要： 目的:研究汉坦病毒汉滩型与汉城型之间能否发生基因重排,以及发生重排的频率和特点。方法:用汉坦病毒汉滩型(HTNV)76-118株与汉城型(SEOV)R22株混合感染Vero-E6细胞,空斑形成实验挑取子代克隆株24株,传代培养后用PCR方法鉴定。结果:发现24株子代病毒(70.83%)的基因组来自76-118株或R22株;1株(4.17%)HTNV型与SEOV型引物扩增均为阳性;1株(4.17%)两型病毒引物扩增均为阴性;4株(16.67%)发生了基因片段重排。结论:证实了汉坦病毒HTNV与SEOV之间可以发生重排。

关键词: 汉坦病毒, 肾综合征出血热, 聚合酶链式反应, 基因重组