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Chines Journal of Vector Biology and Control
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WANG Xue-zhong*; Harold Townson; WANG Pei-yu; et al
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王学忠1;HaroldTownson2;王丕玉1;李菊升1
Abstract: Objective:To compare the sequence difference of the ribosomal DNA second internal transcribed spacer(ITS2) in the different strains of Anopheles minimusin Yunnan.Methods:The PCR amplified rDNA ITS2 sequences,to compare the variation of An.minimus.Results:The different strains of 6 populations were sequenced,which could be separate into Anopheles minimusA and C.Conclusion:The sequencedifference of the rDNA ITS2 would be very useful for the developing a molecular approach to distinguish An.minimusA and C.
摘要: 目的:比较不同地株微小按蚊核糖体DNA(rDNA)内转录间隔2区(ITS2)序列差异。方法:通过对PCR扩增rDNAITS2片段测序,比较其不同地株差异。结果:对6株微小按蚊测序比较,存在有微小按蚊A和C型。结论:rDNAITS2序列差异可用于建立A、C型的分子鉴别方法。
关键词: 微小按蚊, DNA, 核糖体, 第2内转录间隔区
WANG Xue-zhong*; Harold Townson; WANG Pei-yu; et al. Sequence Differences of Second Internal Transcribed Spacer of Ribosomal DNAin Anopheles minimus[J]. Chines Journal of Vector Biology and Control.
王学忠1;HaroldTownson2;王丕玉1;李菊升1. 微小按蚊rDNA内转录间隔2区序列差异[J]. 中国媒介生物学及控制杂志.
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