Chines Journal of Vector Biology and Control ›› 2016, Vol. 27 ›› Issue (5): 450-453.DOI: 10.11853/j.issn.1003.8280.2016.05.007

• Original reports • Previous Articles     Next Articles

Expression profile of aminopeptidase N in different instars of Culex pipiens pallens larvae

GUO Xiu-xia, YANG Pei-pei, CHENG Peng, WANG Hai-fang, LIU Li-juan, ZHANG Chong-xing, WANG Huai-wei, GONG Mao-qing   

  1. Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Key Laboratory of Medicine and Health in Shandong Province, Jining 272033, Shandong Province, China
  • Received:2016-06-16 Online:2016-10-20 Published:2016-10-20
  • Supported by:

    Supported by the National Natural Science Foundation of China (No. 81271877)

淡色库蚊氨肽酶N基因在不同龄期幼虫的表达谱分析

郭秀霞, 杨培培, 程鹏, 王海防, 刘丽娟, 张崇星, 王怀位, 公茂庆   

  1. 山东省医学科学院, 山东省寄生虫病防治研究所, 山东省医药卫生媒介生物学重点实验室, 山东 济宁 272033
  • 通讯作者: 公茂庆,Email:maoqingg@yahoo.com
  • 作者简介:郭秀霞,女,硕士,助理研究员,从事病原生物学研究,Email:guoguoxiua@163.com
  • 基金资助:

    国家自然科学基金(81271877)

Abstract:

Objective To analyze the expression levels of CpAPN in different instars of Culex pipiens pallens. Methods The APN cDNA sequence was identified and sequenced by quantitative Real-time PCR (qRT-PCR), and the relative expression levels of CpAPN were assessed in different instars of larvae using qRT-PCR assays. Results The full-length of CpAPN was 1 383 bp that encoded a protein of 460 amino acid residues. The homology with Cx. pipiens quinquefasciatus (XM001862270.1) was 100%. The qRT-PCR showed that APN was expressed at all stages of Cx. pipiens pallens, with the highest level in the 4th instar and the lowest level in the 3rd instar. There was no significant difference among the 1st, 2nd and 4th instars (F=2.465,P=0.250), but they were all higher than the 3rd instar significantly (F=49.191, P=0.000), which were 3.5 times, 3.7 times, and 4.0 times of the expression levels to the 3rd instar, respectively. Conclusion CpAPN showed significantly different expression levels in different instars of Cx. pipiens pallens. The results provided experimental evidences for revealing the function of CpAPN of the mosquitoes.

Key words: Culex pipiens pallens, Aminopeptidase N, Instar, Larvae, Expression profiling

摘要:

目的 对淡色库蚊不同龄期幼虫中肠氨肽酶N(Aminopeptidase N,APN)的表达水平进行分析。方法 应用反转录-聚合酶链反应(RT-PCR)克隆淡色库蚊APN基因的全长cDNA序列,利用实时荧光定量PCR(qRT-PCR)分析该基因在不同龄期幼虫中肠的表达情况。结果 克隆获得淡色库蚊APN基因,该基因全长1 383 bp,编码460个氨基酸,与GenBank收录的致倦库蚊(XM001862270.1)序列的同源性为100%。qRT-PCR表明CpAPN基因在各个龄期均有表达,其中在4龄期的表达量最高,3龄期最低,1、2和4龄期的表达量差异无统计学意义(F=2.465,P=0.250),与3龄期幼虫的差异有统计学意义(F=49.191,P=0.000),分别为其表达量的3.5、3.7和4.0倍。结论 CpAPN基因在淡色库蚊不同龄期幼虫中的表达水平存在差异,为进一步探讨淡色库蚊APN的功能奠定了基础。

关键词: 淡色库蚊, 氨肽酶N, 龄期, 幼虫, 表达谱

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