Objective To investigate the resistance of Musca domestica to commonly used insecticides in Anshun city, Guizhou province, provide guidance in the control of M. domestica. Methods WHO recommended topical application method was used to determine the resistance levels of M. domestica to four insecticides in Xixiu district, development zone and Pingba county. The experimental data were statistically analyzed by SPSS 17.0 software. Results Musca domestica in Anshun city showed different levels of resistance to four commonly used insecticides. Statistics revealed that the resistance to propoxur could not obtain results by using topical application method, which was significantly different from the other three insecticides. The median lethal dose (LD₅₀) of the adult flies against DDVP, beta-cypemethrin, and deltamethrin were 0.526-0.739, 0.135-0.254, and 0.108-0.176 μg, and the resistance ratios were 175.33-246.33, 7.94-14.94, and 15.43-25.14, respectively; that the resistance to DDVP was the highest. Conclusion Musca domestica in Anshun city have developed resistance to the tested insecticides, especially to propoxur and DDVP. Therefore, in practice, insecticides resistance surveillance should be highlighted for wise use of insecticides.

Objective To determine the status quo of West Nile virus (WNV) infections in animals in Ili, Sinkiang, providing the relevant data for WNV prevention and control in China. Methods One-step real time quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to detect the WNV envelope protein gene from the brain tissues of 70 donkeys and 100 shepherd dogs collected in Ili, Sinkiang. Results Of the brain samples of 70 donkeys and 100 shepherd dogs, 2 were suspected positive for WNV envelope protein gene fragments. However, they were excluded through 3% gel electrophoresis revalidation. Therefore, all tested samples were negative for the WNV envelope protein gene. Conclusion WNV infection is not found in brain tissues of donkeys and shepherd dogs in Ili, Sinkiang.

　　【摘要】　西尼罗病毒（WNV）是一种虫媒病毒，蚊虫尤其是库蚊是其主要传播媒介，它可以导致人和动物严重的神经系统症状。WNV分布广泛，已在非洲、欧洲、澳洲、亚洲、中东、北美等地区引起流行，给发病国家造成了重大危害，引起世界各国研究者的广泛关注。阻断WNV的传入，为防止该病毒在我国的暴发流行，已成为我国卫生工作者的当务之急。加强病毒的监测，熟悉病毒的实验室诊断方法并将其应用在监测工作中就显得尤为重要。现就WNV感染的病原学检测方法加以综述以供参考。