Objective To study the infection of Borrelia burgdorferi among blood donors in Urumqi area. Methods We randomly selected 637 serum samples from blood donors in People's Hospital of Xinjiang Uygur Autonomous Region which were collected from June 2013 to June 2014. All samples were analyzed by IFA to detect the IgM and IgG antibody to B. burgdorferi. Excel 2003 and SPSS 13.0 software were used for data analysis. Results A total of 637 blood donors were tested, 36 cases tested B. burgdorferi IgG positive, infection rate was 5.65%(36/637), 2 cases tested B. burgdorferi IgM positive, recent infection rate was 0.31%(2/637); 601 samples tested negative, percentage was 94.35%(601/637), there was no differences between sexes, ages, nations in B. burgdorferi infection(χ²=0.055, 0.111, 0.044, P=0.814, 0.990, 0.998). Conclusion There were B. burgdorferi infections among blood donors in Urumqi area, suggesting the need to detect the Lyme disease infection in natural epidemic focus of B. burgdorferi, for preventing the infection from spread by blood transfusion.

Objective To investigate the natural progression of Borrelia burgdorferi infection among the human population in some natural foci in Xinjiang, China and the genotypes of B. burgdorferi. Methods In 2006, 119 human subjects, who were randomly selected from 1390 negative cases of 1406 individuals receiving a seroepidemiological survey of B. burgdorferi infection in the summer of 2002, as well as the 16 positive cases in the 2002 survey, were included in the study. Serum samples of each individual were collected in 2002 and 2006 and examined for the IgM and IgG to B. burgdorferi by Western blot, and a questionnaire survey was performed to investigate the frequency of manifestation of Lyme disease. In addition, urine samples were collected from the 135 subjects and examined by nest PCR to amplify 5S-23S rRNA intergenic spacer of B. burgdorferi;some of the PCR positive products were sequenced to determine the genotypes of B. burgdorferi. The PCR detection results were compared with the serological test results. Results Of the 1406 serum samples in 2002, 16 (1.14%) were positive for B. burgdorferi antibodies. In the 16 positive cases, 12 were positive for IgM, 2 were positive for IgG, and 2 were positive for both IgM and IgG. In 2006, 7 (43.75%) of the 16 positive cases in 2002 became seronegative, 4 IgM-positive cases became IgG-positive, and 5 cases remained IgM-positive. Of the 119 negative cases in 2002, 58 (48.74%) became seropositive, including 14 IgM-positive cases, 25 IgG-positive cases, and 19 IgM-and IgG-positive cases, and 2 cases had a confirmed diagnosis of Lyme disease. In 2006, 67 (49.63%) of the 135 subjects were positive for B. burgdorferi antibodies, including 58 newly found cases and 9 cases that remained positive since 2002. Asymptomatic IgG positive seroconversion rate was 34.07% (46/135) (in 2002, 4 IgM-positive cases became IgG-positive; in 2006, 25 negative cases became IgG-positive and 19 negative cases became IgM-and IgG-positive, and 2 cases had a confirmed diagnosis of Lyme disease). Only 3 (2.22%) of the 135 subjects developed Lyme disease. Of the 135 urine samples, 22 (16.30%) had positive results in PCR detection. The sequence analysis of 8 PCR positive products revealed that 7 of them were B. garinii and the other was B. afzelii. Conclusion Most cases of B. burgdorferi infection are asymptomatic among the human population in the natural foci of Xinjiang, and this infection rarely results in Lyme disease. The most and second most frequent genotypes of B. burgdorferi among Xinjiang population are B. garinii and B. afzelii.

　　【Abstract】　Objective　To monitor the population density and seasonal fluctuation of flies at different areas of Hebei, and compare the trapping efficacy of different baits. Methods　Cage trapping method was used in this study. Results　A total of 19 species were caught, belonging to 4 families, 14 genera. Musca domestica was dominant specie. Muscidae were mainly trapped with putrid fish, accounting for 54.22% of the total. Muscidae and Calliphoridae were mostly caught with a mixture of brown sugar and vinegar, accounting for 39.17% and 36.08%, respectively. The season fluctuation tendency of flies caught with two baits was similar, and the peak was all in August.  Conclusion 　According  to  the  surveillance  data,  the  species  controlled  mainly are M.domestica, Chrysomya megacephala, Lucilia sericata, Boettcherisca peregrine and M.stabulans. Environment improvement and the reduction of bleeding sites are the basic measures to control fly density. The putrid fish is worthy to be recommended as a surveillance method of flies density.

　　【Abstract】　Objective　To monitor the population density and seasonal fluctuation of rodents in different residential areas of Hebei province. Methods　The night trapping method and powder trace method were used in this study. Results　There were 6 species caught, belonging to 2 families 4 genera. Rattus norvegicus was the dominant species (60.99%). Mus musculus, Apodemus agrarius, Tscherskia triton and Cricetulus barabensis followed with it, accounting for 25.30%, 9.38%, 2.89% and 0.48% respectively. The dominant species were different in different districts. The results showed that the peaks of population density were in April and October.  Different surveillance methods were used in Langfang, and the average population density of rodents was 0.87%  by night trapping method, but it was only 3.36% by powder trace method. It was 0.83% and 0.67% in Tangshan and Shijiazhuang, respectively. The population density in the country was higher than that in the town. Conclusion　R.norvegicus is the main control target in Hebei province.

Objective In order to obtain basic information of distribution of Dengue viruses vector mosquitoes:Aedes albopictus in north and northwest of Hebei province. Methods Investigation of mosquito larvae adopted small containers method; investigation of mosquito adults adopted mosq-ovitrap, mosq-lamp trap, electro-suction catch method. Results Ae.albopictus was discovered at Shexian county, container index(CI)=34.41, house index(HI)=25.27, breteau index(BI)=4.96. Conclusion Ae.albopictus was discovered at Shexian which is in southwest Hebei province, Ae.albopictus was not found in the city of Chengde in north of Hebei province and the city of Zhangjiakou in northwest of Hebei province.