Objective To investigate the carriage of eight pathogenic microorganisms in Ochotona curzoniae in the Haibei Tibetan Autonomous Prefecture, Qinghai Province, and to provide a scientific basis for the prevention and control of rodent-borne diseases in Haibei Tibetan Autonomous Prefecture. Methods In August 2020, O. curzoniae individuals were captured using the rat trap and rat cage methods at 14 meadow steppe sampling sites in Haiyan County, Qilian County, Gangcha County, and Menyuan Hui Autonomous County in Haibei Tibetan Autonomous Prefecture. Liver, spleen, kidney, and lung tissue samples were collected to extract nucleic acids. The multiplex real-time fluorescence quantitative PCR (qPCR) method was used to detect six disease-causing microorganisms, including Francisella tularensis, Orientia tsutsugamushi, Bartonella spp., Leptospira interrogans, Rickettsia typhi, and Anaplasma phagocytophilum. The multiplex reverse transcription qPCR (RT-qPCR) method was used to detect Hantavirusand Dabie bandavirus. Statistical analyses were performed using SPSS 26.0, and the rates were compared using the Chi-square test and Fisher's exact test. Results In 237 O. curzoniae individuals, 16 (6.75%) were positive for Bartonella and 5 (2.11%) were positive for L. interrogans, with an overall infection rate of 8.44%, and no individuals were positive for the remaining six pathogens. There were significant differences in the positive rates of different pathogenic microorganisms in O. curzoniae (χ2=87.011, P<0.001). Co-infection with Bartonella and L. interrogans was detected in one O. curzoniae individual, yielding a co-infection rate of 0.42%. Conclusions The presence of Bartonella and L. interrogans in O. curzoniae in the meadow environment of Haibei Tibetan Autonomous Prefecture in Qinghai Province suggests that surveillance of pathogens such as plague should be accompanied by strengthened monitoring and prevention of other pathogenic microorganisms, with heightened vigilance against the spread of local rodent-borne diseases.
CHEN Min, GUO Wen-tao, LI Dong-mei
. Investigation of eight pathogenic microorganisms carried by Ochotona curzoniae in Haibei Tibetan Autonomous Prefecture， Qinghai Province， China[J]. Chinese Journal of Vector Biology and Control, 2025
, 36(2)
: 274
-279
.
DOI: 10.11853/j.issn.1003.8280.2025.02.021
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