Investigation

Investigation of the population composition and arbovirus infection of Aedes vector during dengue fever epidemic season in Jinghong, Yunnan Province, China, 2023

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  • 1. Yunnan International Joint Laboratory of Tropical Infectious Diseases, Yunnan International Joint Laboratory of Vector Biology and Control, Yunnan Provincial Key Laboratory of Vector-borne Diseases Control and Research, Yunnan Institute of Parasitic Diseases, Pu'er, Yunnan 665000, China;
    2. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650000, China;
    3. Jinghong Center for Disease Control and Prevention, Jinghong, Yunnan 666100, China

Received date: 2024-08-14

  Online published: 2025-05-09

Supported by

Yunnan Province Science and Technology Talents and Platform Plan (No. 202305AC160012); Science and Technology Basic Resources Investigation Project (No. 2022FY100900)

Abstract

Objective To investigate the population composition and arbovirus infection of Aedes aegypti and Ae. albopictus in Jinghong, Xishuangbanna Dai Autonomous Prefecture (Xishuangbanna Prefecture), Yunnan Province, China during the dengue fever epidemic season in 2023, so as to provide a basis for the prevention and control of dengue fever and other arboviral diseases. Methods During the dengue fever epidemic season in 2023 (from August to October), the change of population composition of Ae. aegypti and Ae. albopictus was monitored in urban areas and Aedes mosquitoes were collected using the double mosquito net method. The infections with Dengue virus, Chikungunya virus, and Zika virus were detected using quantitative PCR (qPCR). The E gene sequences of the viruses were amplified using reverse transcription-PCR (RT-PCR), and a phylogenetic analysis was performed for homological analysis. Results A total of 730 Aedes mosquitoes were collected, of which 548 (75.07%) were Ae. aegypti and 182 (24.93%) were Ae. albopictus. All mosquitoes were divided into 76 groups (21 groups for Ae. albopictus and 55 groups for Ae. aegypti) based on species, sex, and time and location of acquisition. Out of the 76 groups, 11 (14.47%) tested positive for Dengue virus type 1 (DENV-1), yielding an infection rate of 9.52% (2/21) for Ae. albopictus and an infection rate of 16.36% (9/55) for Ae. aegypti, with no significant difference between the two species (χ2=0.574, P=0.449). Zika virus and Chikungunya virus were not detected in any of the mosquitoes. Four DENV-1 E gene sequences (1 from Ae. albopictus and 3 from Ae. aegypti) were successfully obtained from the 11 groups of DENV-1 positive specimens. The sequence similarity of the four sequences was 100%, and all sequences belonged to genotype Ⅰ. The phylogenetic analysis revealed that the strains were closely related to the 2023 Jinghong strain, 2023 Guangzhou strain, 2019 Laos strain, and 2019 Myanmar strain. Conclusions Ae. aegypti is more widely distributed than Ae. albopictus in Jinghong during the dengue fever epidemic season, and DENV-1 is detected in both species. Investigation and research should be enhanced for arboviral infection with Aedes mosquitoes.

Cite this article

WANG Xue-fei, CHEN Li, TAN Li-tao, ZOU Jian-hong, JIANG Jin-yong . Investigation of the population composition and arbovirus infection of Aedes vector during dengue fever epidemic season in Jinghong, Yunnan Province, China, 2023[J]. Chinese Journal of Vector Biology and Control, 2025 , 36(2) : 251 -257 . DOI: 10.11853/j.issn.1003.8280.2025.02.018

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