Taxonomy and Identification

Discovery and identification of Aedes nipponicus in Yanbian Korean Autonomous Prefecture, Jilin Province, China

Expand
  • 1. Department of Veterinary Medicine, College of Agriculture, Yanbian University, Yanji, Jilin 133002, China;
    2. Department of Parasitic Diseases, Yanbian Center for Disease Control and Prevention (Yanbian Health Supervision Institute), Yanji, Jilin 133001, China

Received date: 2024-09-14

  Online published: 2025-05-09

Supported by

National Natural Science Foundation of China (No. 32360886)

Abstract

Objective To identify the species of suspected Aedes mosquito specimens collected from Yanbian Korean Autonomous Prefecture, Jilin Province (Yanbian Prefecture), and to record the local mosquito species. Methods Preliminary morphological identification was performed on suspected adult Aedes mosquitoes captured by human landing collection in the forest in Longjing, Yanbian Prefecture in August 2024. The mitochondrial cytochrome c oxidase subunit Ⅰ (COⅠ) gene was amplified using PCR, and sequencing was performed. The sequences were compared with those in GenBank for similarity, and a phylogenetic tree was constructed using MEGA 6.0 software. Results Five female Aedes mosquitoes were found to share morphological characteristics with Ae. alboniveus Barraud, 1934, Ae. nipponicus LaCasse et Yamaguti, 1948, and Ae. novoniveus Barraud, 1934 in the subgenus Finlaya. TheCOⅠ gene sequences of four randomly selected mosquitoes showed over 99% similarity to known sequences of Ae. nipponicus in GenBank. The phylogenetic tree analysis indicated that they clustered with known sequences of Ae. nipponicus. Conclusions Ae. nipponicus has been discovered in Yanbian Prefecture for the first time, representing a new record of mosquito species in the area. This also provides clear evidence of the distribution of Ae. nipponicus in Jilin Province. The specimens are preserved at the Yanbian Center for Disease Control and Prevention.

Cite this article

LI Ji-xu, WANG Jin-qi, GUAN Yu-tang, LIU Zhe, YIN Guo-lu, XU Ying, XUE Shu-jiang . Discovery and identification of Aedes nipponicus in Yanbian Korean Autonomous Prefecture, Jilin Province, China[J]. Chinese Journal of Vector Biology and Control, 2025 , 36(2) : 246 -250 . DOI: 10.11853/j.issn.1003.8280.2025.02.017

References

[1] Lu BL,Wu HY. Classification and identification of important medical insects of China[M]. Zhengzhou:Henan Science and Technology Press,2003:23-34. (in Chinese) 陆宝麟,吴厚永. 中国重要医学昆虫分类与鉴别[M]. 郑州:河南科学技术出版社,2003:23-34.
[2] Liu GP,Ren QM,Xing AH,et al. Species and fauna distribution of mosquitoes in three provinces of Northeast China[J]. Chin J Hyg Insectic Equip,2012,18(3):215-219. DOI:10.19821/j.1671-2781.2012.03.012.(in Chinese) 刘国平,任清明,邢安辉,等. 东北三省蚊虫种类和区系分布研究[J]. 中华卫生杀虫药械,2012,18(3):215-219. DOI:10.19821/j.1671-2781.2012.03.012.
[3] Hebert PDN,Cywinska A,Ball SL,et al. Biological identifications through DNA barcodes[J]. Proc Roy Soc B Biol Sci,2003,270(1512):313-321. DOI:10.1098/rspb.2002.2218.
[4] Wang X,Tu WC,Huang EJ,et al. Identification of disease-transmitting mosquitoes:Development of species-specific probes for DNA chip assay using mitochondrial COⅠand ND2 genes and ribosomal internal transcribed spacer 2[J]. J Med Entomol,2017,54(2):396-402. DOI:10.1093/jme/tjw195.
[5] Ma Y,Lu L. DNA barcode technology[J]. Chin J Vector Biol Control,2012,23(3):185-190,205. (in Chinese) 马英,鲁亮. DNA条形码技术[J]. 中国媒介生物学及控制杂志,2012,23(3):185-190,205.
[6] Guo YY,Luo L,Song ZY,et al. Mitochondrial DNA sequence analysis of cytochrome oxidase subunitⅠgene from 28 mosquito species in China[J]. Chin J Parasitol Parasit Dis,2017,35(3):280-287. (in Chinese) 郭玉燕,罗雷,宋璋瑶,等. 我国部分地区28种蚊虫的线粒体COⅠ基因序列分析[J]. 中国寄生虫学与寄生虫病杂志,2017,35(3):280-287.
[7] Zhang Q,Zhang F,Gao J,et al. Morphological and DNA barcoding identification of Aedes nipponicus at Henan port[J]. Port Health Control,2021,26(4):57-59,63. DOI:10.3969/j.issn.1008-5777.2021.04.014.(in Chinese) 张勤,张芳,高洁,等. 东瀛伊蚊形态和DNA条形码鉴定[J]. 口岸卫生控制,2021,26(4):57-59,63. DOI:10.3969/j.issn.1008-5777.2021.04.014.
[8] Qu FY,Zhu HM. On revision of the tribe Aedini mosquitoes taxa record in China,with a proposed new classification system (Diptera:Culicidae)[J]. Chin J Parasitol Parasit Dis,2009,27(5):436-447. (in Chinese) 瞿逢伊,朱淮民. 我国伊蚊族蚊类记录的校订及新分类系统的建议(双翅目:蚊科)[J]. 中国寄生虫学与寄生虫病杂志,2009,27(5):436-447.
[9] Lu BL. Fauna Sinica,Insecta vol. 8,Diptera:Culicidae I [M]. Beijing:Science Press,1997:60-259. (in Chinese) 陆宝麟. 中国动物志,昆虫纲第8卷,双翅目,蚊科(上卷)[M]. 北京:科学出版社,1997:60-259.
[10] Su L. Mosquitoes in Northeast China and its adjacent regions[J]. J Norman Bethune Univ Med Sci,1983,9(1):1-9. DOI:10.13481/j.1671-587x.1983.01.001.(in Chinese) 苏龙. 我国东北及其毗邻地区的蚊类[J].白求恩医科大学学报,1983,9(1):1-9. DOI:10.13481/j.1671-587x.1983.01.001.
[11] Su L. The faunal composition and distribution patterns of mosquitoes in Northeast China[J]. J Norman Bethune Univ Med Sci,1983,9(3):9-14. DOI:10.13481/j.1671-587x.1983.03.002.(in Chinese) 苏龙. 我国东北地区蚊类区系成分和分布规律[J].白求恩医科大学学报,1983,9(3):9-14. DOI:10.13481/j.1671-587x.1983.03.002.
[12] Liu GP,Xu ZF,Wang CM,et al. Faunal and ecological studies of mosquitoes along the borders line of northeast China[J]. J Med Pest Control,1999,15(6):281-285. (in Chinese) 刘国平,徐政府,王春梅,等. 东北边境地带蚊虫区系和生态研究[J]. 医学动物防制,1999,15(6):281-285.
[13] Wang G,Li CX,Guo XX,et al. Identifying the main mosquito species in China based on DNA barcoding[J]. PLoS One,2012,7(10):e47051. DOI:10.1371/journal.pone.0047051.
[14] Wang G,Li CX,Guo XX,et al. Phylogenetic relationships in the tribe Aedini (Diptera:Culicidae) based on the cytochrome oxidaseⅠgene[J]. Acta Parasitol Med Entomol Sin,2019,26(2):68-78. DOI:10.3969/j.issn.1005-0507.2019.02.001.(in Chinese) 王刚,李春晓,郭晓霞,等. 利用线粒体COⅠ基因重建伊蚊族(双翅目:蚊科)的系统发育关系(英文)[J]. 寄生虫与医学昆虫学报,2019,26(2):68-78. DOI:10.3969/j.issn.1005-0507.2019.02.001.
[15] Ma F,Zhou QS,Cao HX,et al. Insect intraspecific genetic distance in a forest of Xingangshan,Jiangxi Province[J]. J Environ Entomol,2023,45(4):1027-1037. DOI:10.3969/j.issn.1674-0858.2023.04.20.(in Chinese) 马奉,周青松,曹焕喜,等. 江西新岗山森林昆虫种内遗传距离研究[J]. 环境昆虫学报,2023,45(4):1027-1037. DOI:10.3969/j.issn.1674-0858.2023.04.20.
[16] Wang M,Xie JW,Du YT,et al. Survey of mosquito species and analysis of genetic structure of important mosquito species populations in Wuyi Mountain Nature Reserve[J]. China Trop Med,2024,24(5):499-505. DOI:10.13604/j.cnki.46-1064/r.2024.05.01.(in Chinese) 王明,谢静文,杜雨彤,等. 武夷山自然保护区蚊虫种类调查与重要蚊种种群遗传结构分析[J]. 中国热带医学,2024,24(5):499-505. DOI:10.13604/j.cnki.46-1064/r.2024.05.01.
[17] Li SS,Yang ZX,He YW,et al. Investigation and identification of species of the Culex pipiens complex in Kunming[J]. China Anim Husb Vet Med,2024,51(9):4025-4035. DOI:10.16431/j.cnki.1671-7236.2024.09.030.(in Chinese) 李苏胜,杨振兴,何于雯,等. 昆明尖音库蚊复合组种类的调查与鉴定[J]. 中国畜牧兽医,2024,51(9):4025-4035. DOI:10.16431/j.cnki.1671-7236.2024.09.030.
[18] Zeng XC,Xu X,Yang R,et al. A genetic structure analysis of mtDNACOⅠ gene sequence of Anopheles sinensis populations in Yunnan Province,China[J]. Chin J Vector Biol Control,2021,32(3):265-270. DOI:10.11853/j.issn.1003.8280.2021.03.002.(in Chinese) 曾旭灿,许翔,杨锐,等. 云南省中华按蚊种群mtDNA- COⅠ基因序列的遗传结构分析[J]. 中国媒介生物学及控制杂志,2021,32(3):265-270. DOI:10.11853/j.issn.1003.8280.2021.03.002.
[19] Chen LW,Zhao ZL,Qiu DY,et al. Application of DNA barcoding in classification of rodents at Lanzhou Zhongchuan airport area[J]. Chin J Front Health Quar,2018,41(2):102-105. DOI:10.16408/j.1004-9770.2018.02.008.(in Chinese) 陈利伟,赵志亮,邱德义,等. DNA条形码技术在兰州中川机场鼠类鉴定中的应用[J]. 中国国境卫生检疫杂志,2018,41(2):102-105. DOI:10.16408/j.1004-9770.2018.02.008.
[20] Chen HN,Chu HL,Liu DP,et al. Molecular identification of common cockroaches in China[J]. Chin J Vector Biol Control,2018,29(1):20-22,26. DOI:10.11853/j.issn.1003.8280.2018.01.005.(in Chinese) 陈红娜,褚宏亮,刘大鹏,等. 我国室内常见蜚蠊的分子鉴定研究[J]. 中国媒介生物学及控制杂志,2018,29(1):20-22,26. DOI:10.11853/j.issn.1003.8280.2018.01.005.
[21] Xu W,Wang Y,Wang M,et al. Identification of common sarcosaprophagous flies in the Yangtze River delta by COⅠgene[J]. J Forensic Med,2021,37(5):611-614,626. DOI:10.12116/j.issn.1004-5619.2020.400611.(in Chinese) 徐旺,王禹,王嫚,等. 利用COⅠ基因鉴别长三角地区常见嗜尸性蝇类[J]. 法医学杂志,2021,37(5):611-614,626. DOI:10.12116/j.issn.1004-5619.2020.400611.
[22] Hong HK. Pictorial key to species of mosquitoes in Korea[J]. Report of NIH Korea,1982,19:351-379. (in Korean)
[23] Rising J,Tedesco M,Piontek F,et al. The missing risks of climate change[J]. Nature,2022,610(7933):643-651. DOI:10.1038/s41586-022-05243-6.
[24] Yang K,Wang XH,Lyu S,et al. Impact of global warming on transmission of vector-borne diseases in China[J]. Int J Med Parasit Dis,2006,33(4):182-187,224. DOI:10.3760/cma.j.issn.1673-4122.2006.04.005.(in Chinese) 杨坤,王显红,吕山,等. 气候变暖对中国几种重要媒介传播疾病的影响[J]. 国际医学寄生虫病杂志,2006,33(4):182-187,224. DOI:10.3760/cma.j.issn.1673-4122.2006.04.005.
[25] Greer A,Ng V,Fisman D. Climate change and infectious diseases in North America:The road ahead[J]. CMAJ,2008,178(6):715-722. DOI:10.1503/cmaj.081325.
[26] Yu XH,Xu Y,Wu ZK,et al. Analysis on association with the mosquito-borne diseases and climate factors[J]. Chin J Vector Biol Control,2008,19(1):61-63. DOI:10.3969/j.issn.1003-4692.2008.01.020.(in Chinese) 余向华,徐毅,吴正可,等. 蚊媒传染病气象影响因素分析[J]. 中国媒介生物学及控制杂志,2008,19(1):61-63. DOI:10.3969/j.issn.1003-4692.2008.01.020.
[27] Zhong J,He LH. Advances in research on impacts of meteorological factors on mosquito density[J]. Chin J Vector Biol Control,2015,26(1):95-99. DOI:10.11853/j.issn.1003.4692. 2015.01.028.(in Chinese) 仲洁,何隆华. 气象因素对蚊虫密度影响研究进展[J]. 中国媒介生物学及控制杂志,2015,26(1):95-99. DOI:10.11853/j.issn.1003.4692.2015.01.028.
Outlines

/