Objective To investigate the genotypic characteristics of dengue viruses in Jinghong of Yunnan Province, China, 2023, so as to provide a reference for local prevention and control of dengue fever. Methods From July 13, 2023 to August 4, 2023, serum samples of suspected dengue fever patients were collected at the Xishuangbanna Dai Autonomous Prefecture People's Hospital. Viruses were isolated by cell culture. The Dengue virus envelope gene was amplified via reverse transcription-polymerase chain reaction and sequenced. A phylogenetic tree was constructed using MEGA 11.0 software. Results In 2023, a total of 134 serum samples were collected from patients with suspected dengue fever, of which 127 were positive for Dengue virus nucleic acids, all of which were identified as Dengue virus type 1. Thirteen Dengue virus strains were isolated by cell culture. Based on the envelope gene sequence analysis, they were classified as genotype Ⅰ, which belonged to the same branch as the epidemic strains in Guangzhou (PP563955.1) and Ruili (RL23-83) in 2023, with sequence similarity of 99.90%-100%. The nucleotide similarity with the epidemic strains in Cambodia (OQ678099.1) and Indonesia (ON584400.1) in 2019 was 99.00%-99.10%. Conclusions The dengue fever epidemic in Jinghong in 2023 was mainly caused by dengue virus type 1 (genotype Ⅰ), which is likely to have a similar origin to the strains epidemic in Guangzhou and Ruili. It is recommended that relevant departments should strengthen the surveillance of dengue viruses.
ZHANG Jun-yu, LI Li-hua, CHEN Run, YUAN Li-rong, YANG Wen-zhi, ZHAN Hong-mei, ZENG Fen, YANG Shu-juan, PENG Yan, CHEN Xiao-juan, ZHOU Hong-ning, GAO Guo-yi
. Analysis of genotypic characteristics of dengue viruses in Jinghong， Yunnan Province， China， 2023[J]. Chinese Journal of Vector Biology and Control, 2025
, 36(2)
: 148
-152
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DOI: 10.11853/j.issn.1003.8280.2025.02.002
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