Objective To isolate and identify viruses in Aedes albopictus specimens from Shunyi District, Beijing, China in 2021. Methods The virus was isolated by parallel inoculation of Syrian hamster kidney cell line BHK-21 and Ae. albopictus C6/36 cells with mosquito grinding liquid, and molecular biological identification was carried out for the virus isolates. Results A virus isolate (AalDV-8) was isolated from Ae. albopictus specimens collected from the field in Shunyi District, Beijing in 2021, which could cause C6/36 cell lesion. The nucleotide sequence analysis of the virus genome coding region showed that AalDV-8 virus was a single-stranded DNA virus with a total length of 3 335 nt, encoding 2 non-structural proteins (NS1 and NS2) and 1 capsid protein (VP). The nucleotide (amino acid) sequence lengths of the three proteins were 2 376 nt (791 aa), 1 092 nt (363 aa), and 1 071 nt (356 aa), respectively. The phylogenetic analysis showed that AalDV-8 virus belonged to Brevihamaparvovirus group and was closely related to AalDV-7 virus isolated from Ae. albopictus in Guangzhou, China. Conclusion This is the first time to isolate Brevihamaparvovirus from the field Ae. albopictus collected from in Beijing.
XU Xiu-yan, YAN Ting, LI Jing, ZHU Si-jie, ZHANG Hong-jiang, ZHOU Xiao-jie, TONG Ying, ZHANG Yong
. Isolation and identification of Aedes albopictus Densovirus （Brevihamaparvovirus） in Shunyi District, Beijing, China[J]. Chinese Journal of Vector Biology and Control, 2025
, 36(1)
: 4
-10
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DOI: 10.11853/j.issn.1003.8280.2025.01.002
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