RNA viruses carried by <i>Rhipicephalus microplus</i> in Yinjiang Tujia and Miao Autonomous County, Guizhou Province, China

TANG Ji-xia, HE Xian-hai, WU Sheng-chun, MENG Jiao, CHEN Xing-xing, CHEN Qiu, LIN Zhi-jun, DAI Yi-song, WU Jia-hong, JIANG Jia-fu, SUN Yi, CAO Wu-chun, YU Fu-xun, ZHAN Lin

doi:10.11853/j.issn.1003.8280.2024.05.019

Chinese Journal of Vector Biology and Control >

2024 , Vol. 35 >Issue 5: 621 - 626

DOI: https://doi.org/10.11853/j.issn.1003.8280.2024.05.019

Investigation

RNA viruses carried by Rhipicephalus microplus in Yinjiang Tujia and Miao Autonomous County, Guizhou Province, China

Expand

1. School of Public Health, the Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, Guizhou 561113, China;
2. Dejiang County Agriculture and Rural Affairs Bureau Animal Disease Prevention and Control Center, Tongren, Guizhou 565299, China;
3. NHC Key Laboratory of Pulmonary Immune-related Diseases, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550002, China;
4. School of Public Health, Zunyi Medical University, Zunyi, Guizhou 563006, China;
5. Medical School of Guizhou University, Guiyang, Guizhou 550025, China;
6. Academy of Military Medicine, Academy of Military Sciences, Beijing 100001, China

Received date: 2024-03-25

Online published: 2024-10-18

Supported by

National Natural Science Fund of China (No. 82160633, 81760605); Scientific Research Fund of Guizhou Provincial People's Hospital (No. GPPH-NSFC-2021-17); The High-level and the Innovative Talents of Guizhou Province (No. QKH-GCC [2022] 033-1)

Fold

Abstract

Objective To investigate tick-borne virus threats in Yinjiang Tujia and Miao Autonomous County (Yinjiang County), Guizhou Province, China. Methods Ticks were collected from the body surface of cattle in Yinjiang County. Tick species was identified by morphological characterization and 16S rDNA gene amplification. Viral metagenomic sequencing was performed on the Illumina platform. After quality control and assembly, the virus sequences were aligned with those registered in the National Center of Biotechnology Information. A phylogenetic tree was constructed for the genetic evolution characteristics of the viruses. Results A total of 23 ticks were collected in July, 2023, all of which were identified as Rhipicephalus microplus. According to metagenomic sequencing, the viruses isolated from the ticks belonged to Phenuiviridae, Rhabdoviridae, and Chuviridae and other unclassified viruses. A 5 008 bp nucleotide sequence of Lihan tick virus was assembled in this study. The sequence had the highest homology to the Lihan virus strain (ON812189) isolated from R. microplus in Chongqing in 2019, belonging to a different genetic branch from those of the Rift Valley fever virus (DQ375403) from Egypt and the Severe fever with thrombocytopenia syndrome virus (JQ733567), namely Dabie bandavirus from China. Conclusions Phenuiviridae, Rhabdoviridae, and Chuviridae viruses were found in ticks in Yinjiang County, Guizhou Province. More studies are needed to understand the pathogenicity and public health significance of these tick-borne viruses, especially the Lihan virus found in this study.

Key words： Viral metagenomics; Tick; RNA virus; Guizhou Province; Yinjiang

Cite this article

TANG Ji-xia, HE Xian-hai, WU Sheng-chun, MENG Jiao, CHEN Xing-xing, CHEN Qiu, LIN Zhi-jun, DAI Yi-song, WU Jia-hong, JIANG Jia-fu, SUN Yi, CAO Wu-chun, YU Fu-xun, ZHAN Lin . RNA viruses carried by Rhipicephalus microplus in Yinjiang Tujia and Miao Autonomous County, Guizhou Province, China[J]. Chinese Journal of Vector Biology and Control, 2024 , 35(5) : 621 -626 . DOI: 10.11853/j.issn.1003.8280.2024.05.019

References

[1] Damian D,Maghembe R,Damas M,et al. Application of viral metagenomics for study of emerging and reemerging tick-borne viruses[J]. Vector Borne Zoonotic Dis,2020,20（8）：557-565. DOI：10.1089/vbz.2019.2579.
[2] Shi JM,Hu ZH,Deng F,et al. Tick-borne viruses[J]. Virol Sin,2018,33（1）：21-43. DOI：10.1007/s12250-018-0019-0.
[3] Johnson N,Migné CV,Gonzalez G. Tick-borne encephalitis[J]. Curr Opin Infect Dis,2023,36（3）：198-202. DOI：10.1097/QCO.0000000000000924.
[4] Hawman DW,Feldmann H. Crimean-Congo haemorrhagic fever virus[J]. Nat Rev Microbiol,2023,21（7）：463-477. DOI：10.1038/s41579-023-00871-9.
[5] Meng F,Ding MM,Tan ZZ,et al. Virome analysis of tick-borne viruses in Heilongjiang Province,China[J]. Ticks Tick Borne Dis,2019,10（2）：412-420. DOI：10.1016/j.ttbdis.2018.12.002.
[6] Ma JG,Liu N,Liu ZY,et al. Epidemiology of pathogenic tick-borne viruses in China：A review[J]. Chin J Schistosom Control,2023,35（4）：325-330,348. DOI：10.16250/j.32.1374.2023128.（in Chinese）马静阁,刘宁,刘紫嫣,等. 我国致病性蜱媒病毒流行现状[J]. 中国血吸虫病防治杂志,2023,35（4）：325-330,348. DOI：10.16250/j.32.1374.2023128.
[7] Růžek D,Yakimenko VV,Karan LS,et al. Omsk haemorrhagic fever[J]. Lancet,2010,376（9758）：2104-2113. DOI：10.1016/S0140-6736（10）61120-8.
[8] Xu L,Guo MJ,Hu B,et al. Tick virome diversity in Hubei Province,China,and the influence of host ecology[J]. Virus Evol,2021,7（2）：veab089. DOI：10.1093/ve/veab089.
[9] Chandra S,Harvey E,Emery D,et al. Unbiased characterization of the microbiome and virome of questing ticks[J]. Front Microbiol,2021,12：627327. DOI：10.3389/fmicb.2021.627327.
[10] Liu ZY,Li L,Xu WB,et al. Extensive diversity of RNA viruses in ticks revealed by metagenomics in northeastern China[J]. PLoS Negl Trop Dis,2022,16（12）：e0011017. DOI：10.1371/journal.pntd.0011017.
[11] Yang ZJ,Wang H,Yang SX,et al. Virome diversity of ticks feeding on domestic mammals in China[J]. Virol Sin,2023,38（2）：208-221. DOI：10.1016/j.virs.2023.02.001.
[12] Wang RC,Liu SH,Sun HL,et al. Metatranscriptomics reveals the RNA virome of ixodes persulcatus in the China-North Korea Border,2017[J]. Viruses, 2023,16（1）：62. DOI：10.3390/v16010062.
[13] Xiang YL,Zhou JZ,Yu FX,et al. Characterization of bacterial communities in ticks parasitizing cattle in a touristic location in southwestern China[J]. Exp Appl Acarol,2023,90（1/2）：119-135. DOI：10.1007/s10493-023-00799-y.
[14] Li J,Wang Q,Xia LY,et al. Natural infection of MIV-2 virus in ticks of different regions of China[J]. Acta Parasitol Med Entomol Sin,2021,28（2）：99-103. DOI：10.3969/j.issn.1005-0507.2021.02.006.（in Chinese）李洁,王倩,夏珞媛,等. 我国不同地域蜱类自然感染MIV-2病毒的调查[J]. 寄生虫与医学昆虫学报,2021,28（2）：99-103. DOI：10.3969/j.issn.1005-0507.2021.02.006.
[15] Wang AA,Pang Z,Liu L,et al. Detection and phylogenetic analysis of a novel tick-borne virus in Yunnan and Guizhou provinces,southwestern China[J]. Pathogens,2021,10（9）：1143. DOI：10.3390/pathogens10091143.
[16] Li WY,Li RT,Tang XM,et al. Genomics evolution of Jingmen viruses associated with ticks and vertebrates[J]. Genomics,2023,115（6）：110734. DOI：10.1016/j.ygeno.2023.110734.
[17] Ni XB,Cui XM,Liu JY,et al. Metavirome of 31 tick species provides a compendium of 1801 RNA virus genomes[J]. Nat Microbiol,2023,8（1）：162-173. DOI：10.1038/s41564-022-01275-w.
[18] Xiao P. Prediction of spatial distribution and epidemic analysis of pathogens of Rhipicephalus microplus[D]. Beijing：Beijing University of Chemical Technology,2022. DOI：10.26939/d.cnki.gbhgu.2022.001560.（in Chinese）肖鹏. 微小扇头蜱空间分布及感染病原体流行情况分析[D]. 北京：北京化工大学,2022. DOI：10.26939/d.cnki.gbhgu.2022.001560.
[19] Amarasinghe GK,Ayllón MA,Bào Y,et al. Taxonomy of the order Mononegavirales：Update 2019[J]. Arch Virol,2019,164（7）：1967-1980. DOI：10.1007/s00705-019-04247-4.
[20] Liu MC,Zhang JT,Chen JJ,et al. A global dataset of microbial community in ticks from metagenome study[J]. Sci Data,2022,9（1）：560. DOI：10.1038/s41597-022-01679-7.
[21] Han HM,Zhang YL,Peng J,et al. Overview of medical vector tick organisms and viral infectious diseases[J]. China Port Sci Technol,2021,3（10）：4-11. DOI：10.3969/j.issn.1002-4689.2021.10.001.（in Chinese）韩焕美,张玉磊,彭健,等. 医学媒介生物蜱虫与病毒性传染病概述[J]. 中国口岸科学技术,2021,3（10）：4-11. DOI：10.3969/j.issn.1002-4689.2021.10.001.
[22] Rodino KG,Pritt BS. Novel applications of metagenomics for detection of tickborne pathogens[J]. Clin Chem,2021,68（1）：69-74. DOI：10.1093/clinchem/hvab228.
[23] Li YH,Wang XH,Huang WW,et al. Retracted：Severe fever with thrombocytopenia syndrome virus induces platelet activation and apoptosis via a reactive oxygen species-dependent pathway[J]. Redox Biol,2023,65：102837. DOI：10.1016/j.redox.2023.102837.
[24] Dong ZH,Yang MH,Wang ZD,et al. Human Tacheng tick virus 2 infection,China,2019[J]. Emerg Infect Dis,2021,27（2）：594-598. DOI：10.3201/eid2702.191486.
[25] Teng AY. Spatial distribution and transmission risk prediction of the viral pathogens belonging to the order Bunyavirales in China[D]. Beijing：The Academy of Military Sciences,2022. DOI：10.27193/d.cnki.gjsky.2022.000008.（in Chinese）滕艾颖. 中国布尼亚病毒目病原体的空间分布及传播风险预测研究[D]. 北京：军事科学院,2022. DOI：10.27193/d.cnki.gjsky.2022.000008.
[26] Guo LY,Ma J,Lin JW,et al. Virome of Rhipicephalus ticks by metagenomic analysis in Guangdong,southern China[J]. Front Microbiol,2022,13：966735. DOI：10.3389/fmicb.2022.966735.
[27] Wang J. Investigation of tick-borne pathogens in Nujiang,Yunnan Province[D]. Dali：Dali University,2023. DOI：10.27811/d.cnki.gdixy.2023.000143.（in Chinese）王娟. 云南省怒江州蜱携带病原体的调查研究[D]. 大理：大理大学,2023. DOI：10.27811/d.cnki.gdixy.2023.000143.
[28] Wang WJ,Shin WJ,Zhang BJ,et al. The cap-snatching SFTSV endonuclease domain is an antiviral target[J]. Cell Rep,2020,30（1）：153-163.e5. DOI：10.1016/j.celrep.2019.12.020.
[29] Walker PJ,Freitas-Astúa J,Bejerman N,et al. ICTV virus taxonomy profile：Rhabdoviridae 2022[J]. J Gen Virol,2022,103（6）：001689. DOI：10.1099/jgv.0.001689.
[30] Yang ZJ. Analysis of tick virus community and genetic characteristics of emerging viruses[D]. Zhenjiang：Jiangsu University,2022. DOI：10.27170/d.cnki.gjsuu.2022.000092.（in Chinese）杨子君. 蜱虫病毒群落解析及新发病毒遗传特征研究[D]. 镇江：江苏大学,2022. DOI：10.27170/d.cnki.gjsuu.2022.000092.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References