An investigation of mosquito species and mosquito-borne virus in some areas of Yunnan Province, China, 2023

ZHANG Juan, WANG Ting-ting, PU Shu-wei, FAN Zhi-hong, MAO Meng-ju, JIANG Xue-ying, LI Wen-dong, ZHOU Xiao-mei

doi:10.11853/j.issn.1003.8280.2024.05.018

Chinese Journal of Vector Biology and Control >

2024 , Vol. 35 >Issue 5: 616 - 620

DOI: https://doi.org/10.11853/j.issn.1003.8280.2024.05.018

Investigation

An investigation of mosquito species and mosquito-borne virus in some areas of Yunnan Province, China, 2023

Expand

1. Institute of Disinfection and Vector Control, Yunnan Provincial Center for Disease Control and Prevention, Kunming, Yunnan 650506, China;
2. Honghe Prefecture Center for Disease Control and Prevention, Mengzi, Yunnan 661199, China;
3. Yuxi Center for Disease Control and Prevention, Yuxi, Yunnan 653199, China;
4. Kaiyuan Center for Disease Control and Prevention, Kaiyuan, Yunnan 661699, China

Received date: 2024-04-02

Online published: 2024-10-18

Fold

Abstract

Objective To investigate mosquito species and mosquito-borne viruses in some areas of Yunnan Province, China in 2023, so as to provide evidence for the prevention and control of mosquito-borne infectious diseases. Methods Mosquitoes were collected in Kaiyuan City (in Honghe Hani and Yi Autonomous Prefecture) and Huaning County and Yuanjiang Hani, Yi, and Dai Autonomous County (in Yuxi City) of Yunnan Province from July to August 2023. After species identification, the mosquitoes were examined for various viruses by quantitative PCR. The target virus gene was amplified by conventional PCR, and the virus sequences were analyzed using bioinformatics software. Results A total of 9 060 mosquitoes belonging to 5 species of 4 genera were collected, among which Culex tritaeniorhynchus was the dominant mosquito species, accounting for 50.30% of the total number of mosquitoes caught, followed by Anopheles sinensis, accounting for 33.77%, Cx. pipiens quinquefasciatus, Armigeres subalbatus and Aedes vexans were less, accounting for 5.96%,2.98% and 2.98% respectively. Ten strains of Japanese encephalitis virus were detected in mosquitoes from Kaiyuan City and Huaning County, with a positive rate of 3.31%. No other viruses were detected. According to the NS1 gene-based phylogenetic analysis and nucleotide and amino acid homology analysis of 8 strains of Japanese encephalitis virus from Kaiyuan City and Huaning County, they all belonged to Japanese encephalitis virus genotype Ⅰ. Conclusions In this investigation, Kaiyuan city and Huaning County were dominated by Cx. tritaeniorhynchus, Yuanjiang County was dominted by An. sinensis. Japanese encephalitis virus was for the first time isolated from mosquitoes in Kaiyuan City and Huaning County, indicating that the two places are epidemic areas of Japanese encephalitis, so surveillance and control should be strengthened.

Key words： Mosquito species; Arbovirus; Japanese encephalitis virus; Yunnan Province

Cite this article

ZHANG Juan, WANG Ting-ting, PU Shu-wei, FAN Zhi-hong, MAO Meng-ju, JIANG Xue-ying, LI Wen-dong, ZHOU Xiao-mei . An investigation of mosquito species and mosquito-borne virus in some areas of Yunnan Province, China, 2023[J]. Chinese Journal of Vector Biology and Control, 2024 , 35(5) : 616 -620 . DOI: 10.11853/j.issn.1003.8280.2024.05.018

References

[1] Xia H,Yuan ZM. Discovery and distribution of and response to arbovirus in China over the past seven decades[J]. Chin J Schistosomiasis Control,2023,35（5）：427-436,450. DOI：10.16250/j.32.1374.2023152.（in Chinese）夏菡,袁志明. 70年来我国虫媒病毒发现分布与应对[J]. 中国血吸虫病防治杂志,2023,35（5）：427-436,450. DOI：10.16250/j.32.1374.2023152.
[2] Kang L,Li Y,Cheng G,et al. Infection,transmission,and cross-kingdom immune adaption of arboviruses[J]. Bull Natl Nat Sci Found China,2023,37（6）：1011-1020. DOI：10.16262/j.cnki.1000-8217.2023.06.019.（in Chinese）康乐,李毅,程功,等. 虫媒病毒感染传播及跨界免疫适应机制[J]. 中国科学基金,2023,37（6）：1011-1020. DOI：10.16262/j.cnki.1000-8217.2023.06.019.
[3] Weaver SC,Reisen WK. Present and future arboviral threats[J]. Antiviral Res,2010,85（2）：328-345. DOI：10.1016/j.antiviral.2009.10.008.
[4] Huang YJS,Higgs S,Vanlandingham DL. Emergence and re-emergence of mosquito-borne arboviruses[J]. Curr Opin Virol,2019,34：104-109. DOI：10.1016/j.coviro.2019.01.001.
[5] Zhang HL,Zhang YZ,Yang WH,et al. Mosquitoes of western Yunnan province,China：Seasonal abundance,diversity,and arbovirus associations[J]. PLoS One,2013,8（10）：e77017. DOI：10.1371/journal.pone.0077017.
[6] Zhang HL. Research progress in arboviruses and arboviral diseases in Yunnan Province,China[J]. Chin J Zoonoses,2022,38（12）：1117-1129. DOI：10.3969/j.issn.1002-2694.2022.00.165.（in Chinese）张海林. 云南省虫媒病毒与虫媒病毒病研究进展[J]. 中国人兽共患病学报,2022,38（12）：1117-1129. DOI：10.3969/j.issn.1002-2694.2022.00.165.
[7] Zha B,Zhou HN,Zhang HL,et al. Investigation of mosquitoes and mosquito-borne arboviruses in Yunnan Province,China in 1975-2010[J]. Chin J Vector Biol Control,2012,23（5）：439-444. （in Chinese）查冰,周红宁,张海林,等. 云南省蚊虫媒介与蚊传虫媒病毒研究现状[J]. 中国媒介生物学及控制杂志,2012,23（5）：439-444.
[8] Yang YX,Lin ZR,Na A,et al. Surveillances of the important ecological habits and insecticides resistance to dengue vector Aedes mosquito in Gengma County,China-Myanmar border[J].Chin J Pathog Biol,2023,18（4）：447-450. DOI：10.13350/j.cjpb.230415.（in Chinese）杨耀翔,林祖锐,娜安,等. 中缅边境耿马县登革热媒介伊蚊重要生态习性及抗药性调查[J]. 中国病原生物学杂志,2023,18（4）：447-450. DOI：10.13350/j.cjpb.230415.
[9] Tian H,Guo XX,Yang PP,et al. Species and ecological habits of mosquitoes in Beihu region of Shandong province[J]. Parasitoses Infect Dis,2016,14（3）：125-128. （in Chinese）田华,郭秀霞,杨培培,等. 山东省北湖地区蚊虫种类及生态习性调查[J]. 寄生虫病与感染性疾病,2016,14（3）：125-128.
[10] Liu KN,Ma YL,Wang Q,et al. Epidemiological characteristics of epidemic encephalitis B in Yunnan in 2010-2019[J]. China Prev Med,2021,22（1）：66-69. DOI：10.16506/j.1009-6639.2021.01.013.（in Chinese）刘克娜,马杨岭,王钦,等. 2010—2019年云南省流行性乙型脑炎流行特征分析[J]. 中国预防医学杂志,2021,22（1）：66-69. DOI：10.16506/j.1009-6639.2021.01.013.
[11] Zhu QY,Hu XT,Li QF,et al. Epidemiological characteristics of Japanese encephalitis in Yunnan Province,2005-2015[J]. Chin J Vaccines Immun,2017,23（1）：52-57. DOI：10.19914/j.cjvi.2017.01.013.（in Chinese）朱秋艳,胡筱莛,李琼芬,等. 云南省2005—2015年流行性乙型脑炎流行病学特征分析[J]. 中国疫苗和免疫,2017,23（1）：52-57. DOI：10.19914/j.cjvi.2017.01.013.
[12] Jin AS,Zhong LM. Molecular epidemiology,laboratory and imaging of Japanese encephalitis in Yunnan Province from 2016 to 2022[J]. Chin J Front Health Quar,2024,47（1）：79-83. DOI：10.16408/j.1004-9770.2024.01.019.（in Chinese）金安松,钟莲梅. 2016—2022年云南省流行性乙型脑炎分子流行病学、实验室及影像学检查[J]. 中国国境卫生检疫杂志,2024,47（1）：79-83. DOI：10.16408/j.1004-9770.2024.01.019.
[13] Zhang HL,Zi DY,Gong ZD. Host and vector of Japanese encephalitis virus in Yunnan Province,China[J]. Chin J Prev Vet Med,2000,22（2）：81-83. DOI：10.3969/j.issn.1008-0589.2000.02.001.（in Chinese）张海林,自登云,龚正达. 云南省乙型脑炎病毒宿主和媒介研究[J]. 中国预防兽医学报,2000,22（2）：81-83. DOI：10.3969/j.issn.1008-0589.2000.02.001.
[14] Li J,Zhou HN. Advances of epidemic encephalitis B in Yunnan Province[J]. Chin J Pathog Biol,2008,3（10）：784-787. DOI：10.3969/j.issn.1673-5234.2008.10.021.（in Chinese）李佳,周红宁. 云南省流行性乙型脑炎研究进展[J]. 中国病原生物学杂志,2008,3（10）：784-787. DOI：10.3969/j.issn.1673-5234.2008.10.021.
[15] Deng SZ,Zhang HL,Li JM. Distribution characteristics of mosquito and their natural infection with Japanese encephalitis virus in Yunnan Province[J]. Chin J Vector Biol Control,2009,20（4）：344-348. （in Chinese）邓淑珍,张海林,李金梅. 云南省蚊虫分布特点及自然感染乙型脑炎病毒的研究[J]. 中国媒介生物学及控制杂志,2009,20（4）：344-348.
[16] Li XH,Yu QF,Wu LQ,et al. Epidemiological characteristics of Japanese encephalitis in Yuxi City from 2001 to 2010[J]. J Prev Med Inf,2012,28（7）：567-569. （in Chinese）李秀华,余庆福,吴丽清,等. 2001—2010年玉溪市乙型病毒性脑炎流行病学特征分析[J]. 预防医学情报杂志,2012,28（7）：567-569.
[17] Lyu T,Mou JC,Luo CQ. Epidemiological characteristics of Japanese encephalitis in Honghe Prefecture,Yunnan,2007-2011[J]. J Prev Med Inf,2015,31（4）：259-261. （in Chinese）吕韬,牟建春,罗蟾卿. 2007—2011年云南省红河州流行性乙型脑炎流行特征分析[J]. 预防医学情报杂志,2015,31（4）：259-261.
[18] Roberts A,Gandhi S. Japanese encephalitis virus：A review on emerging diagnostic techniques[J]. Front Biosci （Landmark Ed）,2020,25（10）：1875-1893. DOI：10.2741/4882.
[19] Ling J,Li K,He YW,et al. Isolation and identification of 2 strains of Japanese encephalitis virus from mosquitoes in Tonghai County,Yunnan Province[J]. Chin J Path Biol Parasit Biol,2021,16（5）：525-530. DOI：10.13350/j.cjpb.210506.（in Chinese）凌珏,李凯,何于雯,等. 云南省通海县蚊虫2株乙型脑炎病毒的分离鉴定[J]. 中国病原生物学杂志,2021,16（5）：525-530. DOI：10.13350/j.cjpb.210506.
[20] Zhang J,Li JQ,Feng Y,et al. Identification of Japanese encephalitis virus genotype Ⅰ from mosquitoes in Baoshan,Yunnan[J]. Dis Surveill,2018,33（2）：150-154. DOI：10.3784/j.issn.1003-9961.2018.02.009.（in Chinese）张婧,李加全,冯云,等. 云南省保山市蚊虫中检测到基因Ⅰ型流行性乙型脑炎病毒[J]. 疾病监测,2018,33（2）：150-154. DOI：10.3784/j.issn.1003-9961.2018.02.009.
[21] Sun YJ,Feng Y,Zhang YZ,et al. Molecular characteristics of full-length genomes of four strains of Japanese encephalitis virus newly isolated in Yunnan[J]. Dis Surveill,2015,30（8）：618-623. DOI：10.3784/j.issn.1003-9961.2015.08.004.（in Chinese）孙玉杰,冯云,章域震,等. 云南新分离四株流行性乙型脑炎病毒全基因组序列特征研究[J]. 疾病监测,2015,30（8）：618-623. DOI：10.3784/j.issn.1003-9961.2015.08.004.
[22] Feng Y,Chen WW,Yang WH,et al. Isolation and identification of mosquito-borne viruses in Mile County,Yunnan Province,China in 2009[J]. Chin J Vector Biol Control,2012,23（5）：402-405. （in Chinese）冯云,陈卫武,杨卫红,等. 云南省弥勒县2009年蚊媒病毒分离和鉴定[J]. 中国媒介生物学及控制杂志,2012,23（5）：402-405.
[23] Xie L,Lin AQ,Wang J,et al. An investigation of mosquitoes and mosquito-borne pathogens in the southwest border regions of Yunnan Province,China[J]. Chin J Vector Biol Control,2019,30（3）：264-267. DOI：10.11853/j.issn.1003.8280.2019.03.008.（in Chinese）谢吕,林安琪,王剑,等. 云南省西南边境地区蚊虫及蚊媒病原体调查[J]. 中国媒介生物学及控制杂志,2019,30（3）：264-267. DOI：10.11853/j.issn.1003.8280.2019.03.008.
[24] Wu YQ,Zhou YH,Zhou HN,et al. Investigation of mosquito species composition and their carrying virus rates of important mosquito borne diseases in the part of border areas of China-Vietnam[J]. Chin J Path Biol,2022,17（2）：236-239. DOI：10.13350/j.cjpb.220224.（in Chinese）吴艳琴,周友华,周红宁,等. 中越边境部分地区蚊虫种类及重要虫媒传染病带毒率调查[J]. 中国病原生物学杂志,2022,17（2）：236-239. DOI：10.13350/j.cjpb.220224.
[25] Tian J,Guo XF,Zhou HN,et al. Molecular epidemiological survey of mosquito-borne viral diseases in patients with a fever of unknown origin at the border between China and Myanmar[J].Chin J Path Biol,2021,16（5）：590-595,613. DOI：10.13350/j.cjpb.210519.（in Chinese）田杰,郭晓芳,周红宁,等. 中缅边境地区不明原因发热病人蚊媒病毒性疾病分子流行病学调查[J]. 中国病原生物学杂志,2021,16（5）：590-595,613. DOI：10.13350/j.cjpb.210519.
[26] Li YH,Zhang XW,Cheng G. Arboviruses and biosafety[J]. J Biol,2023,40（6）：1-6. DOI：10.3969/j.issn.2095-1736.2023.06.001.（in Chinese）李雨晗,张櫶文,程功. 虫媒病毒与生物安全[J]. 生物学杂志,2023,40（6）：1-6. DOI：10.3969/j.issn.2095-1736.2023.06.001.
[27] Hou YX,Yao CC,Xu QH,et al. Epidemic situation of important mosquito-borne diseases in China and the progress in vaccine research[J]. Chin J Hyg Insect Equip,2023,29（4）：346-349. DOI：10.19821/j.1671-2781.2023.04.015.（in Chinese）侯银续,姚晨晨,徐庆华,等. 我国重要蚊媒病流行现状和疫苗研究进展[J]. 中华卫生杀虫药械,2023,29（4）：346-349. DOI：10.19821/j.1671-2781.2023.04.015.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References