Objective To assess the diagnostic capacities for malaria of prefecture-level malaria diagnostic reference laboratories of Liaoning Province, China by checking the samples from malaria cases provided by each city from 2015 to 2021 at the provincial reference laboratory, so as to provide a basis for further improving the malaria diagnostic capacity of the centers for disease control and prevention in each city. Methods The blood smear samples were checked under a microscope, and the anticoagulated blood samples from malaria cases were tested using nested PCR and quantitative real time PCR. The Chi-square test and Fisher's exact test were used to compare the detection rate between groups, and to compare the detection rate and reported positive rate. Results A total of 380 samples from malaria cases were checked from 2015 to 2021, in which 270 were positive for Plasmodium falciparum, 20 were positive for P. vivax, 11 were positive for P. malariae, 34 were positive for P. ovale, and 45 were negative. The samples were mainly from Shenyang and Dalian, with a total of 278 samples, accounting for 73.16% of all the samples of the province. The check results showed that the coincidence rate was 95.36% for Shenyang and 92.97% for Dalian, and the average coincidence rate of all the laboratories was 90.13%. The positive rate by nested PCR was 87.63%, and the positive rate by blood smear microscopy was 74.47%, which were significantly different (χ2=46.296, P<0.001). Conclusions Although progress has been made in the capabilities of blood microscopy and nucleic acid detection of prefecture-level centers for disease control and prevention of Liaoning Province, their detection levels are imbalanced, and there is still a large room for improvement. In order to consolidate the malaria elimination achievements, it is necessary to continue to strengthen laboratory personnel training on testing ability, stabilize the testing level, and make sure timely and accurate detection and feedback.
WANG Bo, SUN Ying-wei, LI Xin, WANG Zi-jiang, YU Wei-jun, LEI Lu, YU Wei, MAO Ling-ling
. Review results of malaria samples by Liaoning provincial malaria diagnostic reference laboratories in Liaoning Province, 2015-2021[J]. Chinese Journal of Vector Biology and Control, 2024
, 35(5)
: 581
-586
.
DOI: 10.11853/j.issn.1003.8280.2024.05.013
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