An investigation of <i>Bartonella</i> infections in the natural foci of <i>Meriones unguiculatus</i> plague in Inner Mongolia Autonomous Region, China

LIU Yang, LI Jian-yun, LIU Fang, ZHANG Zhe, ZHANG Jiao

doi:10.11853/j.issn.1003.8280.2024.04.021

Chinese Journal of Vector Biology and Control >

2024 , Vol. 35 >Issue 4: 500 - 507

DOI: https://doi.org/10.11853/j.issn.1003.8280.2024.04.021

Investigation

An investigation of Bartonella infections in the natural foci of Meriones unguiculatus plague in Inner Mongolia Autonomous Region, China

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1. School of Public Health, Baotou Medical College, Baotou, Inner Mongolia 014000, China;
2. Department of Plague Prevention and Control, Inner Mongolia Autonomous Region Center for Disease Control and Prevention, Hohhot, Inner Mongolia 010000, China

Received date: 2024-03-11

Online published: 2024-08-27

Supported by

Research on Key Technologies for Plague Prevention and Control in Inner Mongolia Autonomous Region (No. 2021ZD0006); Investigation and Research on Key Rodent-borne Diseases and Rodent DNA Barcoding in Erenhot Area (No. 2018MS08059); National Key R&D Program of China (No. 2021YFC1200200)

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Abstract

Objective To investigate the distribution and epizootic characteristics of Bartonella in rodents population at the natural foci of Meriones unguiculatus plague in the Inner Mongolia Autonomous Region (Inner Mongolia), China, and study the genetic diversity and evolutionary relationship of Bartonella, so as to provide basic data for the prevention and control of Bartonella infection in humans. Methods In the suburbs of Siziwang Banner and Hangjin Banner of Ulanqab, Inner Mongolia, rodents were collected by night snap trapping. DNA was extracted from rodent liver tissue samples using nucleic acid extraction kits. Polymerase chain reaction (PCR) was used to detect the gltA, ftsZ, and ITS genes. The positive PCR products were sequenced for sequence analysis and phylogenetic analysis for pathogen identification with MEGA 11.0 software. The Fisher's exact test was used to compare the Bartonella infection rates of different rodent species. Results From 2021 to 2022, a total of 175 rodents of seven species (by morphological identification) were collected. The positive rate of Bartonella by gltA gene detection was 42.86% (75/175). These rodents carried six species of Bartonella: B. gerbillinarum, Candidatus B. negeviensis, B. krasnovii, B. grahamii, B. henselae, and B. washoensis. The Bartonella infection rate differed significantly between the 6 species of rodents of M. unguiculatus, Citellus dauricus, Eolagurus luteus, Cricetulus barabensis, Allactaga sibirica, and M. meridianus (P=0.001). Conclusion Bartonella infections are prevalent in this study area, involving a variety of species such as B. grahamii and B. henselae that can infect humans and have host specificity.

Key words： Bartonella; Inner Mongolia; Genetic evolution; Rodent

Cite this article

LIU Yang, LI Jian-yun, LIU Fang, ZHANG Zhe, ZHANG Jiao . An investigation of Bartonella infections in the natural foci of Meriones unguiculatus plague in Inner Mongolia Autonomous Region, China[J]. Chinese Journal of Vector Biology and Control, 2024 , 35(4) : 500 -507 . DOI: 10.11853/j.issn.1003.8280.2024.04.021

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