Objective To detect Songling virus and distribution in ticks collected from Huanan County, Jiamusi, Heilongjiang Province China in 2023.Methods Tick species was identified using morphological methods. The collected tick specimens were examined for Songling virus (SGLV) by using reverse transcription real-time fluorescence quantitative polymerase chain reaction (RT-qPCR). Positive products were sequenced by Sanger sequencing.Results A total of 421 tick specimens were collected, included Dermacentor silvarum (184, 43.71%), Ixodes persulcatus (115, 27.32%), Haemaphysalis japonica (32, 7.60%), H. concinna (43, 10.21%), H. longicornis (7, 1.66%), and 40 unrecognized nymphs (9.50%). Among them, one female H. concinna (ID: 2023JMS365) was tested positive for SGLV nucleic acids by RT-qPCR. The phylogenetic analysis revealed that the nucleotide identencies of the L, M, and S segments of the 2023JMS365 genome were 96.78%, 92.11%, and 97.59% respectively with the SGLV reference strain HLJ1202, and the amino acid identencies were 99.22%, 97.50%, and 93.28%, respectively.Conclusion SGLV was first detected in H. concinna ticks in Huanan County, Jiamusi City.
HOU Ze-ying, LIU Feng-ming, ZHAO Ji-min, LI Chun-yu, LI De, WANG Rui-chen, ZHANG Wei-jia, YAO Xiao-hui, LI Fan, NIE Kai, FU Shi-hong, YIN Qi-kai, CUI Qian-qian, XU Song-tao, LI Xing-zhou, BAO Ming-jia, WANG Huan-yu
. Detection of Songling virus from ticks in Huanan County, Heilongjiang Province, China[J]. Chinese Journal of Vector Biology and Control, 2024
, 35(3)
: 358
-362
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DOI: 10.11853/j.issn.1003.8280.2024.03.018
[1] Mansfield KL,Lv JZ,Phipps LP,et al. Emerging tick-borne viruses in the twenty-first century[J]. Front Cell Infect Microbiol,2017,7：298. DOI：10.3389/fcimb.2017.00298.
[2] Mediannikov O,Fenollar F. Looking in ticks for human bacterial pathogens[J]. Microb Pathog,2014,77：142-148. DOI：10.1016/j.micpath.2014.09.008.
[3] Jia N,Wang JF,Shi WQ,et al. Large-scale comparative analyses of tick genomes elucidate their genetic diversity and vector capacities[J]. Cell,2020,182（5）：1328-1340.e13. DOI：10.1016/j.cell.2020.07.023.
[4] Li F,Liu FM,Feng PP,et al. Investigation of tick-borne viruses in Huanan County,Heilongjiang Province,China,2020[J]. Chin J Vector Biol Control,2022,33（5）：637-641. DOI：10.11853/j.issn.1003.8280.2022.05.005.（in Chinese） 李樊,刘凤明,冯盼盼,等. 黑龙江省桦南县2020年蜱媒病毒调查[J]. 中国媒介生物学及控制杂志,2022,33（5）：637-641. DOI：10.11853/j.issn.1003.8280.2022.05.005.
[5] Boulanger N,Boyer P,Talagrand-Reboul E,et al. Ticks and tick-borne diseases[J]. Med Mal Infect,2019,49（2）：87-97. DOI：10.1016/j.medmal.2019.01.007.
[6] Ji N,Wang N,Liu G,et al. Tacheng tick virus 1 and Songling virus infection in great gerbils （Rhombomysopimus） in Northwestern China[J]. J Wildl Dis,2023,59（1）：138-142. DOI：10.7589/JWD-D-21-00137.
[7] Kazimírová M,Thangamani S,Bartíkova P,et al. Tick-borne viruses and biological processes at the tick-host-virus interface[J]. Front Cell Infect Microbiol,2017,7：339. DOI：10.3389/fcimb.2017.00339.
[8] Hao SY,Ning K,Küz ÇA,et al. Eight years’ advances on Bourbon virus,a tick-born Thogotovirus of the Orthomyxovirus family[J]. Zoonoses （Burlingt）,2022,2（1）：18. DOI：10.15212/zoonoses-2022-0012.
[9] Ma J,Lv XL,Zhang X,et al. Identification of a new Orthonairovirus associated with human febrile illness in China[J]. Nat Med,2021,27（3）：434-439. DOI：10.1038/s41591-020-01228-y.
[10] Li J,Li S,Yang L,et al. Severe fever with thrombocytopenia syndrome virus：A highly lethal Bunyavirus[J]. Crit Rev Microbiol,2021,47（1）：112-125. DOI：10.1080/1040841X.2020.1847037.
[11] Li F,Li JX,Song JD,et al. Novel Orthonairovirus isolated from ticks near China-North Korea border[J]. Emerg Infect Dis,2023,29（6）：1254-1257. DOI：10.3201/eid2906.230056.
[12] Wang QY,Liu FM,Fu SH,et al. Investigation of tick-borne pathogens in Heilongjiang border area[J]. Chin J Exp Clin Virol,2019,33（5）：504-508. DOI：10.3760/cma.j.issn.1003-9279. 2019.05.013.（in Chinese） 王倩莹,刘凤明,付士红,等. 黑龙江边境地区蜱虫携带病原体调查[J]. 中华实验和临床病毒学杂志,2019,33（5）：504-508. DOI：10.3760/cma.j.issn.1003-9279.2019.05.013.
[13] Liu ZY,Li L,Xu WB,et al. Extensive diversity of RNA viruses in ticks revealed by metagenomics in northeastern China[J]. PLoS Negl Trop Dis,2022,16（12）：e0011017. DOI：10.1371/journal.pntd.0011017.
[14] Ali SL,Ali A,Alamri A,et al. Genomic annotation for vaccine target identification and immunoinformatics-guided multi-epitope-based vaccine design against Songling virus through screening its whole genome encoded proteins[J]. Front Immunol,2023,14：1284366. DOI：10.3389/fimmu.2023.1284366.
