Objective To investigate changes in the density, infestation rate, and insecticide resistance of Blattella germanica in Nanjing, China, so as to provide a theoretical basis for scientific and rational use of insecticides.Methods From 2012 to 2022, the density and infestation rate of B. germanica were monitored in the 12 districts of Nanjing, and the resistance of the samples to propoxur, beta-cypermethrin, deltamethrin, acephate, and chlorpyrifos was tested by using the residual film method. The data were processed using the Probit module of SPSS 22.0 software. Spearman’s rank correlation analysis was conducted to correlate year with density, infestation rate, and resistance ratio.Results From 2012 to 2022, the densities of cockroaches were 1.01, 1.62, 2.17, 1.36, 1.21, 0.77, 0.58, 0.39, 0.39, 0.29, and 0.24 individuals/trap, respectively; and the infestation rates were 20.79%, 28.80%, 32.45%, 28.89%, 17.31%, 10.92%, 6.73%, 5.64%, 4.67%, 5.00%, and 3.36%, respectively. The density and infestation rate reached their peaks both in 2014, and decline since 2015, showing significant negative correlations between the year of investigation and the density and infestation rate (ρ=-0.897, P<0.001; ρ=-0.909, P<0.001). Propoxur resistance commenced at a low level in 2015, and the resistance ratio peaked at 4.73 in 2020, showing a significant positive correlation between the year and the resistance ratio (ρ=0.929, P=0.003). Beta-cypermethrin resistance increased to a medium to high level since 2015, and the resistance ratio reached the peak of 40.53 in 2020, showing a significant positive correlation between the year and the resistance ratio (ρ=0.821, P=0.023). Deltamethrin resistance increased to medium to high or even to extremely high levels since 2015, with no significant correlation between the year and the resistance ratio (ρ=0.714, P=0.111). The levels of resistance to chlorpyrifos and acephate were low in general, with no significant correlation between the year and the resistance ratio (ρ=-0.003, P=0.624; ρ=-0.771, P=0.072).Conclusions From 2012 to 2022, the density and infestation rate of B. germanica in Nanjing showed a downward trend, while the resistance to propoxur and beta-cypermethrin increased. The increasing resistance of B. germanica may lead to a rebound in its density and infestation rate, which should be paid more attention to.
ZHANG Yan, SONG Bing-dong, SUN Yan-qun, MIN Yue, CHEN Xue-min, LI Bi-han, SUN Nan, SUN Lu-yao, ZHANG Shou-gang
. Changes in the population density, infestation rate, and insecticide resistance of Blattella germanica in Nanjing, China, 2012-2022[J]. Chinese Journal of Vector Biology and Control, 2024
, 35(3)
: 308
-311
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DOI: 10.11853/j.issn.1003.8280.2024.03.009
[1] Pietri JE,Tiffany C,Liang DS. Disruption of the microbiota affects physiological and evolutionary aspects of insecticide resistance in the German cockroach,an important urban pest[J]. PLoS One,2018,13（12）：e0207985. DOI：10.1371/journal.pone.0207985.
[2] Chehelgerdi M,Ranjbar R. Virulence factors and antibiotic resistance properties of Streptococcus species isolated from hospital cockroaches[J]. 3 Biotech,2021,11（7）：321. DOI：10.1007/s13205-021-02874-w.
[3] Fazeli-Dinan M,Habibi A,Haghi SFM,et al. Determination of susceptibility levels of three different cockroach species including hospitals German cockroach,Blattella germanica L. （Blattodea：Blattellidae）,to common insecticides,cypermethrin,propoxur and fenitrothion[J]. Int J Health Sci （Qassim）,2022,16（4）：13-21.
[4] Cai T,Wang XJ,Liu BR,et al. A cuticular protein,BgCPLCP1,contributes to insecticide resistance by thickening the cockroach endocuticle[J]. Int J Biol Macromol,2024,254：127642. DOI：10.1016/j.ijbiomac.2023.127642.
[5] Gondhalekar AD,Appel AG,Thomas GM,et al. A review of alternative management tactics employed for the control of various cockroach species （Order：Blattodea） in the USA[J]. Insects,2021,12（6）：550. DOI：10.3390/insects12060550.
[6] Chinese Center for Disease Control and Prevention. National vector surveillance implementation program [Z]. Beijing：Chinese Center for Disease Control and Prevention, 2016. （in Chinese） 中国疾病预防控制中心. 全国病媒生物监测实施方案[Z]. 北京：中国疾病预防控制中心,2016.
[7] General Administration of Quality Supervision,Inspection and Quarantine of the People’s Republic of China,Standardization Administration of the People’s Republic of China. GB/T 26352-2010Test methods of cockroach resistance to insecticides-The bioassay methods forBlattella germanica[S]. Beijing：Standards Press of China,2011. （in Chinese） 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会. GB/T 26352-2010蜚蠊抗药性检测方法 德国小蠊生物测定法[S]. 北京：中国标准出版社,2011.
[8] Lee LC,Lee CY. Insecticide resistance profiles and possible underlying mechanisms in German cockroaches,Blattella germanica （Linnaeus） （Dictyoptera：Blattellidae） from Peninsular Malaysia[J]. Med Entomol Zool,2004,55（2）：77-93. DOI：10.7601/mez.55.77_1.
[9] Ghaderi A, Baniardalani M, Basseri HR. Level of pyrethroid-resistance associated with cytochrome P450 expression in German cockroach Blattella germanica （Blattodea： Ectobiidae） in the field collected strains[J]. J Arthropod Borne Dis, 2021, 15（2）： 152-161. DOI：10.18502/jad.v15i2.7484.
[10] Wu XY,Appel AG. Insecticide resistance of several field-collected German cockroach （Dictyoptera：Blattellidae） strains[J]. J Econ Entomol,2017,110（3）：1203-1209. DOI：10.1093/jee/tox072.
[11] Hu IH,Chen SM,Lee CY,et al. Insecticide resistance,and its effects on bait performance in field-collected German cockroaches （Blattodea：Ectobiidae） from Taiwan[J]. J Econ Entomol,2020,113（3）：1389-1398. DOI：10.1093/jee/toaa053.
[12] Wang XJ,Lai SH,Liu F. Cross-resistance of cypermethrin-resistant Blattella germanica to chemical insecticides[J]. Chin J Vector Biol Control,2004,15（3）：178-179. DOI：10.3969/j.issn.1003-4692.2004.03.006. （in Chinese） 王学军,赖世宏,刘峰. 德国小蠊抗性品系对化学杀虫剂交互抗性的研究[J]. 中国媒介生物学及控制杂志,2004,15（3）：178-179. DOI：10.3969/j.issn.1003-4692.2004.03.006.
[13] Hu J,Liu Y,Zhang SH,et al. An analysis of resistance of Blattella germanica to commonly used insecticides in Shenzhen,China[J]. Chin J Vector Biol Control,2021,32（1）：70-73,77. DOI：10.11853/j.issn.1003.8280.2021.01.014. （in Chinese） 胡静,刘阳,张韶华,等. 广东省深圳市德国小蠊对常用杀虫剂的抗药性分析[J]. 中国媒介生物学及控制杂志,2021,32（1）：70-73,77. DOI：10.11853/j.issn.1003.8280.2021.01.014.
[14] Li TQ,Wu YY,Liu QM,et al. Surveillance results of the insecticide resistance of Blattella germanica in Zhejiang Province of China,2018[J]. Chin J Vector Biol Control,2022,33（4）：462-465. DOI：10.11853/j.issn.1003.8280.2022.04.003. （in Chinese） 李天奇,吴瑜燕,刘钦梅,等. 浙江省2018年德国小蠊抗药性监测结果分析[J]. 中国媒介生物学及控制杂志,2022,33（4）：462-465. DOI：10.11853/j.issn.1003.8280.2022.04.003.
[15] Kovacic P,Somanathan R. Propoxur：A novel mechanism for insecticidal action and toxicity[J]. Rev Environ Contam Toxicol,2012,218：141-150. DOI：10.1007/978-1-4614-3137-4_4.
[16] Hou YX,Wang F,An Z,et al. Resistance of Blattella germanica to commonly used insecticides in Wuhu,Anhui Province,China,2022[J]. Chin J Vector Biol Control,2023,34（5）：612-616. DOI：10.11853/j.issn.1003.8280.2023.05.005. （in Chinese） 侯银续,王斐,安洲,等. 安徽省芜湖市2022年德国小蠊对常用卫生杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志,2023,34（5）：612-616. DOI：10.11853/j.issn.1003.8280. 2023.05.005.
[17] Hu IH,Tzeng HY,Chen ME,et al. Association of CYP4G19 expression with gel bait performance in pyrethroid-resistant German cockroaches （Blattodea：Ectobiidae） from Taiwan[J]. J Econ Entomol,2021,114（4）：1764-1770. DOI：10.1093/jee/toab104.
[18] Lee SH,Choe DH,Rust MK,et al. Reduced susceptibility towards commercial bait insecticides in field German cockroach （Blattodea：Ectobiidae） populations from California[J]. J Econ Entomol,2022,115（1）：259-265. DOI：10.1093/jee/toab244.
