Objective To investigate the prevalence of five types of infections with zoonotic bacterial pathogens (Bartonella, Borrelia burgdorferi, Anaplasma phagocytophilum, Leptospira, and Babesia) in pet dogs in Changzhi, Shanxi Province, China.Methods Anticoagulant blood samples from pet dogs were collected to detect Babesia (by conventional PCR) and Bartonella, Bor. burgdorferi, A. phagocytophilum, and Leptospira (by quantitative PCR). Positive PCR products were sequenced for analysis. The infection rates between groups were compared using Fisher's exact test.Results A total of 98 blood samples from pet dogs were collected, with 4 of 98 samples (4.08 %) positive for Bartonella, 3 of 98 (3.06 %) positive for Bor. burgdorferi, 7 of 98 (7.14 %) positive for A. phagocytophilum, 3 of 98 (3.06 %) positive for Babesia, and none positive for Leptospira. There was no significant difference in the detection rate of any pathogen between dogs of different sexes, ages, and vaccination status (Fisher's exact test, P>0.05). Two pet dogs had mixed infections, with Bartonella and A. phagocytophilum in one dog and Bor. burgdorferi and A. phagocytophilum in the other one. Two samples positive for Babesia were sequenced successfully, and phylogenetic analysis showed that they were both Bab. gibsoni.Conclusions Multiple zoonotic bacterial pathogens are prevalent in pet dogs in Changzhi, Shanxi Province, which may infect humans and animals. It is necessary to strengthen the management of pet dogs and their epidemic diseases in this area.
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