Objective To investigate the repellent effects of 19 botanical compounds derived from Oleaceae and Rosaceae on Aedes albopictus by testing their effective protection time alone and in combination in laboratory.Methods Under laboratory conditions, the selected compounds showed the highest repellency rates against Ae. albopictus at a volume concentration of 0.01 among 19 compounds through the close proximity response assay. Next, 2-phenylethanol and dihydrojasmone were tested using the Arm in cage test for their repellency to Ae. albopictus at volume concentrations of 0.064, 0.128, and 0.256. Then 2-phenylethanol (concentration, 0.256) and dihydrojasmone (concentration, 0.256) were mixed at volume ratios of 1:1, 1:4, 4:1, 1:7, 7:1, 1:15, and 15:1 for Ae. albopictus repellency testing. Finally, the mosquito repellents of 2-phenylethanol (concentration, 0.256) and dihydrojasmone (concentration, 0.256) were prepared and tested at volume ratios of 1:15 and 15:1 as the main effective components. The repellent activity of different compounds or combinations against Ae. albopictus was compared by using the independent samples t-test and one-way analysis of variance.Results The repellent rates of 2-phenylethanol and dihydrojasmone both reached 100 % by close proximity response assay. When used alone, 2-phenylethanol and dihydrojasmone showed repellent effects on Ae. albopictus at concentrations of 0.064, 0.128, and 0.256, and their effective protection time differed significantly from that of the control group (all P<0.001). At the concentration of 0.256, the repelling time was significantly different between the mixtures of 2-phenylethanol and dihydrojasmone at different volume ratios (F=21.457, P<0.001). For repellents with 2-phenylethanol and dihydrojasmone mixed at 15:1 and 1:15, the effective protection time reached 4.50 h and 6.25 h, respectively.Conclusion The combination of 2-phenylethanol and dihydrojasmone shows significant repellent effects on Ae. albopictus, which can be further developed.
ZHOU Chu-chu, LIU Jing, HE Hong-yang, FENG Bo
. The repellent effects of 2-phenylethanol and dihydrojasmone on Aedes albopictus[J]. Chinese Journal of Vector Biology and Control, 2024
, 35(2)
: 150
-155
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DOI: 10.11853/j.issn.1003.8280.2024.02.004
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