Objective To evaluate and compare the efficacy of polydimethylsiloxane liquid film against the larvae and pupae of Culex pipiens quinquefasciatus, Aedes albopictus, and Ae. aegypti at different instars under laboratory conditions.Methods Thirty samples each of larvae of different instars and pupae were selected and placed in water containing the liquid film (1 ml/m2). The growth,development,and death of the larvae or pupae were observed in a laboratory environment or in an artificial incubator at different temperatures. The data were organized and analyzed using Excel 2016 and SPSS 27.0 softwares. The survival curves of larvae or pupae exposed to the liquid film between different groups were compared using the log-rank test. The length of larvae was compared using the independent samples t-test.Results After exposure to the 1 ml/m2 liquid film,the median lethal times for the larvae at Ⅰ-Ⅱinstars of Cx. pipiens quinquefasciatus, Ae. albopictus, and Ae. aegypti were 2.67, 7.04, and 6.45 d, respectively; for the larvae at Ⅲ-Ⅳ instars, the values were 0.53,1.64, and 1.71 d, respectively; and for the pupae, the values were 96.68,115.54, and 109.77 min, respectively. After 7 days of rearing, the mean larval lengths for Cx. pipiens quinquefasciatus, Ae. albopictus, and Ae. aegypti exposed to the liquid film were (3.28±0.40), (3.17±0.61), and (3.21±0.47) mm, respectively, which were significantly shorter than their respective control groups (all P<0.001). Across the temperatures of 20, 25, 30, and 35 ℃, the mortality rates of larvae in the liquid film groups increased with rising temperatures:the mortality rates on the first day were 66.67 %, 71.11 %, 91.11 %, and 100 % for Cx. pipiens quinquefasciatus, respectively; 8.89 %, 30.00 %, 55.56 %, and 81.11 % for Ae. albopictus, respectively; and 14.44 %, 25.56 %, 56.67 %, and 75.56 % for Ae. aegypti, respectively.Conclusions The polydimethylsiloxane liquid film can well control the larvae and pupae of the three mosquito species and impede larval growth and development, and high temperatures can enhance the effectiveness. The polydimethylsiloxane liquid film is suitable for controlling various types of mosquitoes.
ZHANG Ming-wei, DUAN Jin-hua, DENG Hui, LU Rui-peng, CHEN Zong-jing, LIN Li-feng
. Laboratory efficacy of polydimethylsiloxane liquid film against three mosquito species[J]. Chinese Journal of Vector Biology and Control, 2024
, 35(2)
: 133
-137
.
DOI: 10.11853/j.issn.1003.8280.2024.02.001
[1] Lin LF.The current situation of emerging and re-emerging vector-borne infectious diseases and the prevention and control measures of vectors[J].Chin J Hyg Insect Equip,2020,26(3):193-196.DOI:10.19821/j.1671-2781.2020.03.001.(in Chinese) 林立丰.新发与再发病媒传染病流行现状与病媒生物防控对策[J].中华卫生杀虫药械,2020,26(3):193-196.DOI:10.19821/j.1671-2781.2020.03.001.
[2] Antonio-Nkondjio C,Sandjo NN,Awono-Ambene P,et al.Implementing a larviciding efficacy or effectiveness control intervention against malaria vectors:Key parameters for success[J].Parasit Vectors,2018,11(1):57.DOI:10.1186/s13071-018-2627-9.
[3] Ngrenngarmlert W,Sukkanon C,Yaicharoen R,et al.Physical influence on larvicidal and pupicidal activity of the silicone-based monomolecular film[J].Acta Trop,2016,162:239-244.DOI:10.1016/j.actatropica.2016.07.012.
[4] Mbare O,Lindsay SW,Fillinger U.Aquatain®mosquito formulation (AMF) for the control of immature Anopheles gambiae sensu stricto and Anopheles arabiensis:Dose-responses,persistence and sub-lethal effects[J].Parasit Vectors,2014,7:438.DOI:10.1186/1756-3305-7-438.
[5] Webb CE,Russell RC.A laboratory investigation of the mosquito control potential of the monomolecular film Aquatain®mosquito formula against immature stages of Aedes aegypti and Culex quinquefasciatus[J].J Am Mosq Control Assoc,2009,25(1):106-109.DOI:10.2987/08-5750.1.
[6] Zhang ZD,Jin SQ,Wen HY,et al.Control efficacy of polydimethylsiloxane liquid film on Aedes albopictus larvae under laboratory conditions[J].Chin J Hyg Insect Equip,2022,28(1):1-5.DOI:10.19821/j.1671-2781.2022.01.001.(in Chinese) 张振东,金姝青,温和毅,等.聚二甲基硅氧烷液态防蚊膜在实验室条件下对白纹伊蚊幼虫防治效果的研究[J].中华卫生杀虫药械,2022,28(1):1-5.DOI:10.19821/j.1671-2781.2022.01.001.
[7] Du X.Efficacy of anti-mosquito film against Culex pipiens pallens and Aedes albopictus larvae in laboratory[J].Chin J Hyg Insect Equip,2020,26(5):417-422.DOI:10.19821/j.1671-2781.2020.05.005.(in Chinese) 杜雪.防蚊水膜对淡色库蚊和白纹伊蚊幼虫的实验室防治效果观察[J].中华卫生杀虫药械,2020,26(5):417-422.DOI:10.19821/j.1671-2781.2020.05.005.
[8] Wang T,Song CL,Xu F,et al.A study of the control effect of a new mosquito control film on Aedes albopictus[J].Chin J Vector Biol Control,2020,31(5):555-558.DOI:10.11853/j.issn.1003.8280.2020.05.011.(in Chinese) 王唐,宋灿磊,许峰,等.一种新型灭蚊膜对白纹伊蚊控制效果的研究[J].中国媒介生物学及控制杂志,2020,31(5):555-558.DOI:10.11853/j.issn.1003.8280.2020.05.011.
[9] Yamada H,Maiga H,Bimbile-Somda NS,et al.The role of oxygen depletion and subsequent radioprotective effects during irradiation of mosquito pupae in water[J].Parasit Vectors,2020,13(1):198.DOI:10.1186/s13071-020-04069-3.
[10] Wigglesworth VB.The function of the anal gills of the mosquito larva[J].J Exp Biol,1933,10(1):16-26.DOI:10.1242/jeb.10.1.16.
[11] Silberbush A,Abramsky Z,Tsurim I.Dissolved oxygen levels affect the survival and developmental period of the mosquito Culex pipiens[J].J Vector Ecol,2015,40(2):425-427.DOI:10.1111/jvec.12186.
[12] Dawood AFD,Baz MM,Ibrahim MI.Influence of AquatainTM,a monomolecular surface film on surface tension for controlling the filarial vector Culex pipiens(Diptera:Culicidae)[J].Heliyon,2020,6(10):e05314.DOI:10.1016/j.heliyon.2020.e05314.
[13] Lee SJ,Kim JH,Lee SC.Effects of oil-film layer and surfactant on the siphonal respiration and survivorship in the fourth instar larvae of Aedes togoi mosquito in laboratory conditions[J].Sci Rep,2018,8(1):5694.DOI:10.1038/s41598-018-23980-5.
[14] Ren DS,Zhou ZJ.An overview on the application and safety assessment of polydimethylsiloxane[J].Chin J Food Hyg,2011,23(2):181-185.DOI:10.13590/j.cjfh.2011.02.005.(in Chinese) 任东升,周志俊.聚二甲基硅氧烷应用及安全性评估概况[J].中国食品卫生杂志,2011,23(2):181-185.DOI:10.13590/j.cjfh.2011.02.005.
[15] Thomas IM.The reactions of mosquito larvae to regular repetitions of shadows as stimuli[J].Aust J Biol Sci,1950,3(1):113-123.DOI:10.1071/bi9500113.
