Objective To investigate the species composition, density, seasonal variation, and virus-carrying status of mosquitoes in Taizhou, Zhejiang Province, China, since the implementation of the new vector surveillance program in 2017-2022, so as to provide a scientific basis for the control of mosquito vectors and the early warning of mosquito-borne diseases.Methods Surveillance sites were set up in 9 counties (cities and districts) of Taizhou, and the light trapping method and Breteau index (BI) method were used to perform ecological surveillance of adult and larval mosquitoes from April to November every year. From June to August in 2022, adult mosquitoes were collected in Luqiao District, and quantitative real-time PCR was used to detect six viruses including Dengue virus, Japanese encephalitis virus, West Nile virus, Chikungunya virus, Yellow fever virus, and Sindbisvirus. SPSS 20.0 software was used to perform a statistical analysis of surveillance data.Results In 2017-2022, a total of 4 800 mosquito trap lamp·nights were deployed in Taizhou, and 109 160 female mosquitoes were captured in total, with a mean mosquito density of 22.74 mosquitoes/lamp·night, among which there were 76 799 Culex tritaeniorhynchus mosquitoes, accounting for 70.35%, followed by 20 724 Cx. pipiens pallens/quinquefasciatus mosquitoes, accounting for 18.98%. June to September was the peak period of mosquito activity. In different habitats, livestock shed (livestock farm) had the highest mosquito density of 92.91 mosquitoes/lamp·night. Cx. tritaeniorhynchus was the dominant species in livestock shed (livestock farm), accounting for 83.08%, and Cx. pipiens pallens/quinquefasciatus was the dominant species in residential area and other habitats, with a composition ratio of >60%. There was a significant difference in the composition ratio of mosquito species between different habitats (χ2=60 286.496, P<0.001). A total of 94 523 households were surveyed for mosquito larvae surveillance, with a mean BI of 16.94. All the monthly average BI from April to November were greater than 5. Positive stagnant water was mainly observed in idle containers (bowls, bottles, jars, and tanks), accounting for 54.88%, and the highest breeding rate of Aedes (37.37%) was observed in tires/waste tires. A total of 1 050 female Cx. pipiens pallens/quinquefasciatus mosquitoes and 1 140 female Ae. albopictus mosquitoes were detected for above 6 viruses, and all mosquitoes were negative for the six viruses.Conclusions Cx. pipiens pallens/quinquefasciatus is the dominant mosquito species in the residential area in Taizhou, and there is a relatively high density of mosquitoes in livestock shed (livestock farm) where the dominant mosquito species is Cx. tritaeniorhynchus. There is a risk of dengue fever transmission from April to November. It is necessary to strengthen comprehensive mosquito control measures focusing on environmental management, so as to prevent the transmission and epidemic of imported or indigenous mosquito-borne diseases..
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