Objective To analyze the whole-genome sequences of Hantavirus in rodent lung samples collected from Zhoushan Islands in Zhejiang province, China in 2021.Methods Nested PCR was used to amplify the Hantavirus nucleic acid in Hantavirus positive rodent lung samples. The sequence was assembled by SeqMan 7.1.0.44 in DNAStar, and the sequence alignment and phylogenetic analysis were performed by MEGA 7.0.Results The whole-genome sequences of three Hantavirus strains were successfully determined, all of which were identified as Seoul (SEOV) S3 subtype, with high homology with strains from Rui'an in Zhejiang, Liaoning, and Shandong. They showed high homology in nucleotide and amino acid sequences and consistent immune epitopes of 2 glycoproteins and 4 nucleoproteins with the vaccine strain Z37.Conclusion This study first reported the whole-genome sequences of Hantavirus isolated from Zhoushan Islands in Zhejiang Province, which provides a reference for the study of the epidemic characteristics of hemorrhagic fever with renal syndrome and the virus evolution patterns in this area.
SHU Ji-wei, CHEN Xu-hui, TAN Qi-long, HUANG Zhe, YE Ling, WANG Zhi-ping, HUANG Mao-wen, LI Shi-bo, CHEN Qian, ZHANG Tong-jie
. Whole-genome sequence analysis of three strains of Hantavirus in Zhoushan Islands, Zhejiang Province, China[J]. Chinese Journal of Vector Biology and Control, 2023
, 34(5)
: 607
-611
.
DOI: 10.11853/j.issn.1003.8280.2023.05.004
[1] Kim KA,Choi GH,Jang ES,et al. Epidemiology and treatment status of Hepatitis C virus infection among people who have ever injected drugs in Korea:A prospective multicenter cohort study from 2007 to 2019 in comparison with non-PWID[J]. Epidemiol Health,2021,43:e2021077. DOI:10.4178/epih.e2021077.
[2] Diagne MM,Dieng I,Granjon L,et al. Seoul Orthohantavirus in wild black rats,Senegal,2012-2013[J]. Emerg Infect Dis,2020,26（10）:2460-2464. DOI:10.3201/eid2610.201306.
[3] Hepojoki J,Strandin T,Lankinen H,et al. Hantavirus structure-molecular interactions behind the scene[J]. J Gen Virol,2012,93（Pt 8）:1631-1644. DOI:10.1099/vir.0.042218-0.
[4] Heuser E,Drewes S,Trimpert J,et al. Pet rats as the likely reservoir for human Seoul Orthohantavirus infection[J]. Viruses,2023,15（2）:467. DOI:10.3390/v15020467.
[5] Chen XH,Tan QL,Shu JW,et al. Molecular epidemiological analysis of Hantavirus in an outbreak of hemorrhagic fever with renal syndrome in Daishan county,Zhejiang province,China[J]. Chin J Vector Biol Control,2022,33（5）:706-709. DOI:10. 11853/j.issn.1003.8280.2022.05.017.（in Chinese） 陈旭辉,谭启龙,舒纪为,等. 浙江省岱山县一起肾综合征出血热疫情汉坦病毒分子流行病学分析[J]. 中国媒介生物学及控制杂志,2022,33（5）:706-709. DOI:10.11853/j.issn.1003. 8280.2022.05.017.
[6] Geng YZ,Tian J,Liu Y,et al. Genetic subtypes and distribution of Seoul hanta virus in Liaoning province[J]. Chin J Public Health,2012,28（12）:1594-1596. DOI:10.11847/zgggws-2012-28-12-15.（in Chinese） 耿英芝,田疆,刘芸,等. 辽宁省汉城型汉坦病毒基因亚型分析[J]. 中国公共卫生,2012,28（12）:1594-1596. DOI:10. 11847/zgggws-2012-28-12-15.
[7] Luo XH,Huang SJ,Chen LF,et al. An investigation on the infection status and genotype of Hantavirus carried by rodents in Yuyao city of Zhejiang province[J]. Prev Med,2016,28（8）:789-791,795. DOI:10.19485/j.cnki.issn1007-0931.2016.08.008.（in Chinese） 罗学辉,黄邵军,陈立锋,等. 余姚市鼠形动物携带汉坦病毒及M基因分析[J]. 预防医学,2016,28（8）:789-791,795. DOI:10.19485/j.cnki.issn1007-0931.2016.08.008.
[8] Chai NM,Tan QL,Shu JW,et al. Investigation and analysis of a clustered epidemic of hemorrhagic fever with renal syndrome[J]. Chin J Health Lab Technol,2023,33（1）:95-97,101. （in Chinese） 柴能明,谭启龙,舒纪为,等. 一起肾综合征出血热聚集性疫情调查分析[J]. 中国卫生检验杂志,2023,33（1）:95-97,101.
[9] Dong XQ,Shi LM,Wang YQ,et al. Serological detection and genotype analysis of Hantavirus isolated from patients with suspected hemorrhagic fever with renal syndrome （HFRS） in the Nanjing metropolitan area[J]. Chin J Zoonoses,2022,38（4）:322-326. DOI:10.3969/j.issn.1002-2694.2022.00.037.（in Chinese） 董晓庆,石利民,王雅倩,等. 南京都市圈肾综合征出血热疑似病例血清学检测及基因亚型分析[J]. 中国人兽共患病学报,2022,38（4）:322-326. DOI:10.3969/j.issn.1002-2694.2022. 00.037.
[10] Hu HF,Du H,Yi HY,et al. Advances in antiviral treatment and vaccine development of hemorrhagic fever with renal syndrome[J]. Infect Dis Inf,2020,33（3）:198-201. DOI:10.3969/j.issn.1007-8134.2020.03.002.（in Chinese） 胡海峰,杜虹,伊宏煜,等. 肾综合征出血热抗病毒治疗及疫苗研发进展[J]. 传染病信息,2020,33（3）:198-201. DOI:10.3969/j.issn.1007-8134.2020.03.002.
[11] Cheng LF,Zhang FL,Xu ZK. Progress in HFRS vaccine research[J]. Chin J Viral Dis,2011,1（6）:459-462. DOI:10.16505/j.2095-0136.2011.06.007.（in Chinese） 程林峰,张芳琳,徐志凯. 肾综合征出血热新型疫苗研究进展[J]. 中国病毒病杂志,2011,1（6）:459-462. DOI:10.16505/j.2095-0136.2011.06.007.
[12] Fang EY. Progress in research on viral hemorrhagic fevers[J]. Chin J Biol,2019,32（6）:707-712. DOI:10.13200/j.cnki.cjb.002643.（in Chinese） 房恩岳. 病毒性出血热的研究进展[J]. 中国生物制品学杂志,2019,32（6）:707-712. DOI:10.13200/j.cnki.cjb.002643.
[13] You J,Zhang Z. Research progress of Hantavirus vaccine[J]. Chin J Hyg Insect Equip,2022,28（1）:90-93. DOI:10.19821/j.1671-2781-2022.01.025.（in Chinese） 尤佳,张振. 汉坦病毒疫苗的研究进展[J]. 中华卫生杀虫药械,2022,28（1）:90-93. DOI:10.19821/j.1671-2781.2022.01.025.
[14] Wang JW,Zhao YJ,Song Z,et al. Research progress on structural protein of Hantavirus and biological activity of its epitope[J]. Mod J Anim Husb Vet Med,2007（4）:60-63. DOI:10.3969/j.issn.1672-9692.2007.04.041.（in Chinese） 王建文,赵玉军,宋卓,等. 汉坦病毒结构蛋白及其表位生物学活性的研究进展[J]. 现代畜牧兽医,2007（4）:60-63. DOI:10.3969/j.issn.1672-9692.2007.04.041.
[15] Liu SW,Zhang YN,Xiao F,et al. Isolation and complete genome sequence analysis of two Seoul Orthohantavirus strains isolated from Gaoan city,Jiangxi province[J]. Chin J Zoonoses,2022,38（2）:102-107. DOI:10.3969/j.issn.1002-2694.2022.00.023.（in Chinese） 刘师文,张艳妮,肖芳,等. 江西省高安市两株汉城病毒分离鉴定及全基因组序列分析[J]. 中国人兽共患病学报,2022,38（2）:102-107. DOI:10.3969/j.issn.1002-2694.2022.00.023.
