Resistance to three pyrethroid insecticides and knockdown resistance gene mutations in <i>Aedes albopictus</i> in Guiyang,Guizhou Province,China

ZHANG Yan, WANG Dan, ZHOU Jing-zhu, SHI Wei-fang, LUO Xiao-long, KONG Xue-xue, YU Hao, GUAN Yu-wei, HU Yong, LIANG Wen-qin

doi:10.11853/j.issn.1003.8280.2023.05.003

Chinese Journal of Vector Biology and Control >

2023 , Vol. 34 >Issue 5: 600 - 606

DOI: https://doi.org/10.11853/j.issn.1003.8280.2023.05.003

Experimental Study

Resistance to three pyrethroid insecticides and knockdown resistance gene mutations in Aedes albopictus in Guiyang,Guizhou Province,China

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1. School of Public Health, the Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, Guizhou 550025, China;
2. Department of Vector Surveillance, Experimental Center, Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China

Received date: 2023-03-23

Online published: 2023-10-27

Supported by

Plan Project of the Science and Technology in Guizhou Province (No. Qian Ke He [2022] General 178, Qian Ke He [2020]4Y169); Scientific Research Team of Guizhou Provincial Infectious Disease Prevention and Control Talent Base (No. RCJD2107)

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Abstract

Objective To evaluate the level of resistance of Aedes albopictus to pyrethroid insecticides in Guiyang,China,and identify mutations in the knockdown resistance gene, so as to provide a scientific basis for the control of Ae. albopictus in the region.Methods From July to August 2022,the larvae of Ae. albopictus were captured at different locations of Guiyang and then reared to F1-F2 generations in the laboratory. The resistance to pyrethroid insecticides was determined by using the dipping method for larvae and the World Health Organization tube method for adult mosquitoes. The genomic DNA of individual adult Ae. albopictus mosquito was extracted for PCR amplification and sequencing to detect knockdown resistance gene mutations. The Chi-square test was used to analyze the frequency differences of mutant genes between groups.Results The median lethal concentrations of deltamethrin, permethrin, and beta-cypermethrin against Ae. albopictus larvae in Guiyang were 0.559, 0.021, and 0.012 mg/L, respectively;and the resistance ratios were 433.33, 46.67, and 16.44, respectively. The mortality rates of adult Ae. albopictus mosquitoes exposed to 0.03% deltamethrin, 0.4% permethrin, and 0.08% beta-cypermethrin were all <80%. No mutations were detected at the 1016, 1532, and 1534 loci in the sensitive population of Ae. albopictus. Knockdown resistance gene mutations were found at all the three loci in the natural population of Ae. albopictus. There were two alleles at 1016 locus, which were wild-type GTA (V) (76.35%) and mutant GGA (G) (23.65%), with no statistically significant differences in frequency of mutant genes between the sensitive and resistant phenotypes (χ²=1.810, P=0.178); There were three genotypes at 1016 locus: wild-type homozygous V/V (58.78%), wild/mutant heterozygous V/G (35.14%),and mutant homozygous G/G (6.08%). There were two alleles at 1532 locus, which were wild-type ATC (I) (99.83%) and mutant ACC (T) (0.17%),with two genotypes of wild-type homozygous I/I (99.66%) and wild/mutant heterozygous I/T (0.34%). There were two alleles at 1534 locus, namely wild-type TTC (F) (48.48%) and mutant TCC (S) (51.52%),with no statistically significant difference in frequency of mutant genes between the sensitive and resistant phenotypes (χ²=0.603, P=0.437); There were three genotypes at 1534 locus: wild-type homozygous F/F (8.11%), wild/mutant heterozygous F/S (80.74%),and mutant heterozygous S/S (11.15%).Conclusions Larval and adult Ae. albopictus mosquitoes in Guiyang have developed medium-to-high resistance to the three pyrethroid insecticides and mutations in the knockdown resistance gene,but with no significant association between the mutations and the resistant phenotypes. The level of resistance in the region should be continuously monitored to guide the scientific and rational use of insecticides to effectively control mosquitoes and delay the evolution of insecticide resistance.

Key words： Aedes albopictus; Insecticide resistance; Knockdown resistance gene; Resistance phenotype

Cite this article

ZHANG Yan, WANG Dan, ZHOU Jing-zhu, SHI Wei-fang, LUO Xiao-long, KONG Xue-xue, YU Hao, GUAN Yu-wei, HU Yong, LIANG Wen-qin . Resistance to three pyrethroid insecticides and knockdown resistance gene mutations in Aedes albopictus in Guiyang,Guizhou Province,China[J]. Chinese Journal of Vector Biology and Control, 2023 , 34(5) : 600 -606 . DOI: 10.11853/j.issn.1003.8280.2023.05.003

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