ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Distribution of knockdown resistance genotypes in Aedes albopictus in Nanning, Guangxi Zhuang Autonomous Region, China, 2022
Received date: 2023-03-20
Online published: 2023-08-17
Supported by
Scientific Research Project of Health Commission of Guangxi Zhuang Autonomous Region(Z-A20221268);Scientific Research Project of Health Commission of Guangxi Zhuang Autonomous Region(Z20201224)
Objective: To investigate knockdown resistance (kdr) genotypes of Aedes albopictus which is the major vector of dengue in Nanning, so as to understand their insecticide resistance levels and provide evidence for scientific control of Ae. albopictus. Methods: Ae. albopictus larvae were collected using the dip method in Nanning and raised to adults in the laboratory. After morphological identification, Ae. albopictus mosquitoes were soaked in 75% ethanol and stored at -20 ℃. DNA was extracted from individual mosquitoes using the magnetic bead-based method. The partial fragments of the voltage-gated sodium channel (VGSC) gene were amplified by PCR and sequenced for alignment with the Basic Local Alignment Search Tool on the platform of National Center for Biotechnology Information. DNAStar 7.1 was used to analyze single-site and multiple-site mutations in the confirmed VGSC gene of Ae. albopictus mosquitoes. Results: A total of 175 Ae. albopictus mosquitoes were examined in 2022, obtaining 350 sequences (about 400 bp in length). Mutations were detected at the 1016 and 1534 loci but not at the 1532 locus of the VGSC gene. At the 1016 locus, there were two alleles (wild-type V [148, 78.28%] and mutant G [49, 21.72%]) and three genotypes (wild-type homozygote V/V [126, 72.00%], mutant homozygote G/G [27, 15.43%], and wild-type/mutant heterozygote V/G [22, 12.57%]). At the 1532 locus, there was one allele (wild-type I [175, 100%]) and one genotype (wild-type homozygote I/I [175, 100%]). At the 1534 locus, there were three alleles (wild-type F [51, 16.86%], mutant S [116, 36.29%], and mutant C [135, 46.85%]) and six genotypes (wild-type homozygote F/F [8, 4.57%], wild/mutant heterozygotes F/S [21, 12.00%] and F/C [22, 12.57%], mutant heterozygote S/C [64, 36.57%], and mutant homozygotes S/S [21, 12.00%] and C/C [39, 22.29%]). Conclusion: VGSC gene mutations are frequent in Ae. albopictus in Nanning. Attention should be paid to the insecticide resistance levels of Ae. albopictus for scientific, standardized, and efficient use of insecticides.
Xue LI, Feng LING, Shu-lin WEI, Zhi-qiang QU, Shu-qin DIAO, Yan-cui HUANG, Mi-fang LUO . Distribution of knockdown resistance genotypes in Aedes albopictus in Nanning, Guangxi Zhuang Autonomous Region, China, 2022[J]. Chinese Journal of Vector Biology and Control, 2023 , 34(4) : 480 -484 . DOI: 10.11853/j.issn.1003.8280.2023.04.007
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