Investigation on mosquito species and <i>Plasmodium</i> sporozoite infection in <i>Anopheles</i> mosquitoes in Laiza city, Myanmar, 2018

TIAN Peng, SUN Xiao-dong, DUAN Kai-xia, XU Yan-chun, ZHOU Yao-wu, GUO Xiang-rui, LI Shi-gang, LIN Zu-rui

doi:10.11853/j.issn.1003.8280.2023.03.022

Chinese Journal of Vector Biology and Control >

2023 , Vol. 34 >Issue 3: 412 - 416

DOI: https://doi.org/10.11853/j.issn.1003.8280.2023.03.022

Investigation

Investigation on mosquito species and Plasmodium sporozoite infection in Anopheles mosquitoes in Laiza city, Myanmar, 2018

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1. Yunnan Institute of Parasitic Disease Prevention and Control Malaria Control Department, Yunnan Key Laboratory of Insect Borne Infectious Disease Prevention and Control, Yunnan Key Technology Innovation Team for Insect Borne Infectious Disease Prevention and Control, Pu'er, Yunnan 665000, China;
2. Yingjiang County Center for Disease Control and Prevention, Yingjiang, Yunnan 679300, China

Received date: 2023-02-14

Online published: 2023-06-16

Supported by

National Natural Science Foundation of China (No. 81960374)

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Abstract

Objective To investigate mosquito species and Plasmodium sporozoite infection in Anopheles mosquitoes in Laiza city, Myanmar, and provide a basis for local malaria control and prevention.Methods Four residential sites were selected in Laiza city (within 2 km from the border), and five human houses were selected at each site for capturing mosquitoes. Mosquitoes were collected with a light trap each inside and outside every house overnight for three days, twice per month from July to September, 2018. Mosquito species were determined by morphological identification. The positive rate of Plasmodium sporozoite infection in Anopheles mosquitoes was examined by using nested PCR, with five Anopheles mosquitoes per group.Results A total of 2 041 mosquitoes of four genera were collected, including 815 (39.93%) Culex mosquitoes, 16 (0.78%) Aedes mosquitoes, 453 (22.20%) Armigeres mosquitoes, and 757 (37.09%) Anopheles mosquitoes. There were 10 Anopheles species, in which An. minimus was the local dominant species, accounting for 85.87% (650/757), followed by An. culicifacies (5.02%). A total of 667 An. minimus mosquitoes in 135 groups were examined by PCR. Among them, 11 groups were positive for the target fragment of P. vivax, all labeled as An. minimus, with a detection rate of 8.15%. In the positive 11 groups, at least one An. minimus mosquito in each group carried P. vivax sporozoites, and the minimum Plasmodium sporozoite infection rate in An. minimus mosquitoes was 1.69% (11/650). The minimum infection rates of Zhatugong and Chunmuweng sites were 1.74% and 1.89%, respectively, without statistical difference (χ²=0.012, P=0.912). The infection rate was zero at Jiyangka and Penlongyang sites.Conclusions An. minimus was the dominant Anopheles species in Laiza city, Myanmar, which was the main vector for local malaria transmission, with a high rate of Plasmodium sporozoite infection.

Key words： Myanmar; Laiza city; Mosquito; Anopheles; Plasmodium sporozoite; Nested PCR

Cite this article

TIAN Peng, SUN Xiao-dong, DUAN Kai-xia, XU Yan-chun, ZHOU Yao-wu, GUO Xiang-rui, LI Shi-gang, LIN Zu-rui . Investigation on mosquito species and Plasmodium sporozoite infection in Anopheles mosquitoes in Laiza city, Myanmar, 2018[J]. Chinese Journal of Vector Biology and Control, 2023 , 34(3) : 412 -416 . DOI: 10.11853/j.issn.1003.8280.2023.03.022

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