Lethal effects of recombinant CYP307A1 RNAi Chlorella by feeding on Aedes albopictus

HUANG Xiao-dan, XIAO Sa, HE Chang-hao, LI Zhi-jie, ZHANG Xiu-xia, LI Ya-jun, FEI Xiao-wen, DENG Xiao-dong

doi:10.11853/j.issn.1003.8280.2023.03.010

Chinese Journal of Vector Biology and Control >

2023 , Vol. 34 >Issue 3: 336 - 343

DOI: https://doi.org/10.11853/j.issn.1003.8280.2023.03.010

Experimental Study

Lethal effects of recombinant CYP307A1 RNAi Chlorella by feeding on Aedes albopictus

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1. Key Laboratory of Molecular Biology, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, Hainan 571199, China;
2. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science/Hainan Tropical Agricultural Resources Research Institute, Haikou, Hainan 570216, China

Received date: 2022-10-23

Online published: 2023-06-16

Supported by

National Natural Science Foundation of China (No. 31870344, 82260669); Key R&D Projects in Hainan Province (No. ZDYF2022SHFZ314); Hainan Postgraduate Innovative Scientific Research Project (No. Hys2020-364)

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Abstract

Objective To investigate the effects of silencing the ecdysone synthesis pathway enzyme gene CYP307A1 of Aedes albopictus on the growth and development of Aedes larvae, and to provide new ideas for the prevention and control of mosquito-borne infectious diseases.Methods An RNAi expression vector targeting CYP307A1 was constructed using RNAi technology. Chlorella was used as the recipient for electrotransformation. The selected recombinant Chlorella strains were fed to Ae. albopictus larvae. WPS 2020 software was used to pool data on the mortality, pupation rate, emergence rate, body length, and quantitative real-time PCR results of the expression of CYP307A1 gene in larvae. One-way analysis of variance was performed on the mean values of all groups using SPSS 25. Origin 2019 and WPS 2020 were used to make charts.Results The 300-mosquito experiment showed that the mortality rates of larvae in the control groups fed with filtered water, feed, wild Chlorella, and Chlorella transferred with pMaa7/IR vector were 1.50%, 0.17%, 0.83%, and 8.00%, respectively; the mortality rate of larvae fed with recombinant CYP307A1 RNAi Chlorella was 80.33%, which was statistically higher than that of the control groups (P<0.05). The pupation rate and emergence rate of Aedes larvae fed with feed were 99.83% and 98.50%, respectively, while those of the group fed with recombinant CYP307A1 RNAi Chlorella were 18.50% and 98.50%, respectively. The pupation rate and emergence rate with recombinant CYP307A1 RNAi Chlorella were statistically lower than those of the control group (P<0.05). The expression of the CYP307A1 gene in Aedes larvae fed with the recombinant Chlorella was decreased by 79.00% compared with the control group (P<0.01).Conclusion Recombinant CYP307A1 RNAi Chlorella has anti-mosquito function, which shows promise in the field of biological mosquito control.

Key words： Aedes albopictus; CYP307A1; RNA interference; Chlorella

Cite this article

HUANG Xiao-dan, XIAO Sa, HE Chang-hao, LI Zhi-jie, ZHANG Xiu-xia, LI Ya-jun, FEI Xiao-wen, DENG Xiao-dong . Lethal effects of recombinant CYP307A1 RNAi Chlorella by feeding on Aedes albopictus[J]. Chinese Journal of Vector Biology and Control, 2023 , 34(3) : 336 -343 . DOI: 10.11853/j.issn.1003.8280.2023.03.010

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