Objective To investigate the population distribution, virus-carrying status, and virus gene characteristics of host animals for Hantavirus in some areas of Zhoushan Islands, Zhejiang province, China, and to provide evidence for prevention and control of hemorrhagic fever with renal syndrome (HFRS).Methods Small mammals were captured using the night trapping method in Dinghai and Daishan areas of Zhoushan Islands, followed by species identification. The rodent density and species composition were analyzed using the Chi-square test. The small mammal’ lungs were collected to detect the nucleic acid of Hantavirus by quantitative real-time PCR. The M gene was amplified by nested PCR and sequenced for genotyping and homology analysis.Results In Dinghai and Daishan areas of Zhoushan Islands in 2021, the average rodent density was 3.42%, with 1.71% in the wild and 6.16% in residential areas. Six species of small mammals were captured. The dominant species was Apodemus agrarius in the wild and Rattus norvegicus in residential areas. The density of rodents was statistically different between Daishan Island, Yushan Island, and Dinghai Island (χ2=108.468, P<0.001), between the wild and residential areas (χ2=68.935, P<0.001), and between different species (χ2=37.749, P<0.001). Hantavirus RNA was detected in 13 small mammals lung specimens, with a positive rate of 6.70%. The M fragments of three Hantavirus RNA extracts were successfully amplified and sequenced. The homology of the three M fragments was 96.90%-97.10%, belonging to Seoul virus (SEOV, S3 subtype).Conclusions There are HFRS foci with R. norvegicus as the source of infection in Zhoushan Islands, where SEOV S3 subtype is the epidemic strain. Environmental changes may alter natural foci.
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