Detection of knockdown resistance gene mutations in <i>Aedes albopictus</i> in Yuzhou, Henan province, China

MU Qun-zheng, HUA Dong-dong, LI Wen-yu, ZHOU Xin-xin, LUN Xin-chang, LI Gui-chang, WANG Jun, SONG Xiu-ping, LIU Qi-yong, MENG Feng-xia

doi:10.11853/j.issn.1003.8280.2023.03.004

Chinese Journal of Vector Biology and Control >

2023 , Vol. 34 >Issue 3: 303 - 307

DOI: https://doi.org/10.11853/j.issn.1003.8280.2023.03.004

Experimental Study

Detection of knockdown resistance gene mutations in Aedes albopictus in Yuzhou, Henan province, China

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1. State Key Laboratory of Infectious Disease Prevention and Control, Department of Vector Biology and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Vector Surveillance and Management, Beijing 102206, China;
2. School of Public Health, Shandong University, Ji'nan, Shandong 250000, China;
3. Ningxia Center for Disease Prevention and Control, Yinchuan, Ningxia 750000, China;
4. Daxing District Center for Disease Control and Prevention of Beijing, Beijing 102600, China

Received date: 2023-02-13

Online published: 2023-06-16

Supported by

National Science and Technology Major Project of China (No. 2018ZX101010002-002)

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Abstract

Objective To investigate the knockdown resistance gene mutations of Aedes albopictus in Yuzhou, Henan province, China, and to provide a basis for the control of Ae. albopictus.Methods The double-layered mosquito net method was used to capture mosquitoes in Yuzhou in mid-September of 2020 and 2022. Mosquito species was identified based on morphological characteristics. The DNA of Ae. albopictus mosquitoes was extracted for knockdown resistance gene detection by sequencing.Results A total of 80 Ae. albopictus mosquitoes (40 in each year) were captured. The sequencing results showed that three knockdown resistance gene loci were found mutant in Ae. albopictus. The 1016 locus mutated from valine (Val, V) encoded by the codon GTA to glycine (Gly, G) encoded by GGA. The 1532 locus mutated from ATC-encoded isoleucine (Ile, I) to ACC-encoded threonine (Thr, T). Mutations at 1534 were most frequent, from TTC-encoded phenylalanine (Phe, F) to TCC-encoded serine (Ser, S) or Leu (Leu, L) encoded by TTA/CTC. There were 10 genotypes in total for the three mutant sites. For all the three mutant sites, wild-type homozygotes were predominant, followed by wild/mutant heterozygotes and then mutant homozygotes. There were a total of 11 combination genotypes: the frequency of wild/mutant heterozygous combination genotypes at single loci was 55.00%, which was highest; the frequency of wild homozygous combination genotypes at three loci was 28.75%; the frequency of wild/mutant heterozygous genotypes at two loci was 8.75%; and the frequency of mutant homozygous genotypes at single loci was 7.50%, which was lowest.Conclusions It was the first time to carry out knockdown resistance gene mutation detection in Ae. albopictus in Yuzhou, discovering that the mutations were frequent and complex, which requires further research in this area.

Key words： Aedes albopictus; Knockdown resistance gene; Yuzhou

Cite this article

MU Qun-zheng, HUA Dong-dong, LI Wen-yu, ZHOU Xin-xin, LUN Xin-chang, LI Gui-chang, WANG Jun, SONG Xiu-ping, LIU Qi-yong, MENG Feng-xia . Detection of knockdown resistance gene mutations in Aedes albopictus in Yuzhou, Henan province, China[J]. Chinese Journal of Vector Biology and Control, 2023 , 34(3) : 303 -307 . DOI: 10.11853/j.issn.1003.8280.2023.03.004

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