Objective To investigate the genetic diversity, genetic differentiation, and phylogenetic relationship of Culex pipiens pallens populations in Shangdong, China.Methods Cx. pipiens pallens populations were collected in Heze, Dezhou, Liaocheng, Qingdao, Yantai, and Rizhao, Shandong province from September 2020 to September 2022. Genomic DNA was extracted from a single female adult mosquito. The mitochondrial cytochrome c oxidase subunit Ⅰ (mtDNA-COⅠ) gene sequences were amplified by polymerase chain reaction and sequenced afterwards. The acquired sequences were compared with those in the Basic Local Alignment Search Tool on the GenBank. BioEdit 7.0 was used to compare and analyze the sequencing results. DnaSP v6 was used to analyze the genetic diversity of populations. Arlequin 3.5 was used to calculate the genetic differentiation coefficient and gene flow of populations. PopART 1.7 was used to construct the haplotype network (TCS network).Results A total of 423 sequences of 603 bp were obtained from 6 Cx. pipiens pallens populations in Shandong province. The average content of A+T bases was 69.2%, which was consistent with the AT bias of mitochondrial DNA. There were eight haplotypes in the haplotype analysis, of which haplotype H01 was the dominant haplotype. The results of mtDNA-COⅠ sequence analysis showed that the population had rich genetic diversity. The results of molecular variance analysis indicated that the genetic differentiation of Cx. pipiens pallens mainly came from within the population; there was some genetic differentiation among some populations. The results of neutrality test showed that the populations of Cx. pipiens pallens in Liaocheng and Heze experienced expansion recently.Conclusions The mtDNA-COⅠ gene can be used as a molecular marker to study the genetic diversity of Cx. pipiens pallens populations. The genetic development of Qingdao population is special compared with other geographical populations.
ZANG Chuan-hui, LI Li-ming, LIU Shuo, GONG Mao-qing, WANG Wen-qian, WANG Yi-ting, LOU Zi-wei, LEI Jing-jing, CHENG Peng, LIU Hong-mei
. Genetic diversity of Culex pipiens pallens populations in Shandong province, China based on the mtDNA-COⅠgene[J]. Chinese Journal of Vector Biology and Control, 2023
, 34(3)
: 298
-302
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DOI: 10.11853/j.issn.1003.8280.2023.03.003
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