Experimental Study

Genetic diversity and genetic structure of Lasiopodomys brandtii populations in three regions of Inner Mongolia, China

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  • 1. State Key Laboratory of Infectious Disease Prevention and Control, Department of Vector Biology and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

Received date: 2023-01-05

  Online published: 2023-06-16

Supported by

Key Project of National Natural Science Foundation of China (No. 32090023)

Abstract

Objective To analyze the genetic diversity of nine batches of Lasiopodomys brandtii rodents from three sample plots (New Barag Right banner, East Ujimqin banner, and Xilinhot city) of the Inner Mongolia Autonomous Region (Inner Mongolia), China by using 16 pairs of microsatellite primers.Methods A total of 275 L. brandtii rodents were trapped using snaps or cages in May, July, and September of 2021. DNA was extracted from them with magnetic beads, followed by PCR amplification with 16 pairs of fluorescent-labeled microsatellite primers. The microsatellite data were obtained using GeneMarker. The genetic diversity and structure of L. brandtii populations were analyzed using GenAIEx 6.5, Arlequin (version 30), and structure 2.3.4.Results A total of 472 alleles were detected by using 16 pairs of microsatellite primers. The average polymorphic information content was 0.821 3. The average Shannon’s information index was 1.812. Observed heterozygosity ranged from 0.478 to 0.951. Expected heterozygosity ranged from 0.575 to 0.891. According to cluster analysis and principal component analysis, the nine L. brandtii populations could be divided into four groups by month. The genetic diversity index of the four groups was May > July > September. The analysis of molecular variance showed that the variation among individuals (88.51%) was much greater than that among the groups (5.04%) and that among the populations (6.45%). The mantel test showed no significant correlation between genetic distance and geographical distance of L. brandtii (R2=0.001 6).Conclusions The L.brandtii populations sampled in Inner Mongolia had high genetic diversity. The genetic variation of L. brandtii populations was mainly observed among individuals. Seasonal variation could be the main factor affecting the genetic structure of L. brandtii populations.

Cite this article

LIU Yu-qiu, LU Liang, LIU Peng-bo, ZHAO Ning, LI Gui-chang, LI Dong-mei, SONG Xiu-ping, WANG Jun, LIU Qi-yong . Genetic diversity and genetic structure of Lasiopodomys brandtii populations in three regions of Inner Mongolia, China[J]. Chinese Journal of Vector Biology and Control, 2023 , 34(3) : 291 -297 . DOI: 10.11853/j.issn.1003.8280.2023.03.002

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