Investigation

Investigation of Babesia bigemina carried by ticks in Shibing county, Guizhou province, China

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  • 1. School of Public Health, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, Guizhou 550025, China;
    2. Medical School of Guizhou University, Guiyang, Guizhou 550025, China;
    3. Central Laboratory, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550002, China;
    4. Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550001, China;
    5. Academy of Military Medicine, Academy of Military Sciences, Beijing 100001, China

Received date: 2022-10-26

  Online published: 2023-04-26

Supported by

National Natural Science Foundation of China (No. 82160633,81760605);High-level and Innovative Talents of Guizhou Province (No. QKH-GCC[2022]033-1)

Abstract

Objective To investigate the infection of Babesia bigemina in ticks at Shibing county in Guizhou province of China. Methods Ticks were collected from the body surface of free-range cattle in Shibing from 2021 to 2022. The 16S rDNA and cytochrome c oxidase subunit Ⅰ(COX1) gene detection techniques were used to identify the tick species after preliminary morphological classification. The samples were initially screened with Piroplasma 18S rDNA gene, followed by PCR verification and sequencing with B. bigemina apical membrane antigen-1 (AMA-1) gene. The resulting sequences were aligned using BLAST at the National Center for Biotechnology Information (NCBI) website. Phylogenetic trees were constructed by using the neighbor-joining method to understand the genetic evolution characteristics of tick-borne B. bigemina in Shibing. Results In this study, 615 ticks were collected and assigned to 2 genera and 4 species, including Rhipicephalus microplus, R. haemaphysaloides, Haemaphysalis longicornis, and H. kitaokai. Nucleic acid fragments of B. bigemina were detected in R. microplus. The sequencing result showed that the AMA-1 gene sequence was closely related to the B. bigemina detected from bovine blood in the Philippines and the tick of Hyalomma anatolicum in Sudan, and the 18S rDNA sequence was most closely related to B. bigemina in India. Conclusions The parasitic ticks on the body surface of free-range cattle in Shibing carry the nucleic acid fragments of B. bigemina, suggesting infection of the vector ticks in Shibing with B. bigemina. Our results provide reference data for the prevention and control of tick-borne protozoonosis in this area.

Cite this article

WU Sheng-chun, MENG Jiao, HUANG Jian-sheng, YU Fu-xun, WU Jia-hong, YANG Guang-hong, JIANG Jia-fu, SUN Yi, CAO Wu-chun, ZHAN Lin . Investigation of Babesia bigemina carried by ticks in Shibing county, Guizhou province, China[J]. Chinese Journal of Vector Biology and Control, 2023 , 34(2) : 254 -261 . DOI: 10.11853/j.issn.1003.8280.2023.02.019

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