Experimental Study

A comparative proteomics analysis of beta-cypermethrin resistance in Aedes albopictus population in Changping, Beijing, China

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  • 1. School of Public Health, Cheeloo College Medicine, Shandong University, Ji'nan, Shangdong 250102, China;
    2. State Key Laboratory of Infectious Disease Prevention and Control, Department of Vector Biology and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

Received date: 2022-11-28

  Online published: 2023-04-26

Supported by

National Science and Technology Major Project of China (No. 2018ZX10101002-002);National Natural Science Foundation of China (No. 82073615)

Abstract

Objective To perform bioinformatics analysis of differentially expressed proteins between beta-cypermethrin-resistant and -sensitive individuals of Aedes albopictus population in Changping, Beijing, China, to study the metabolic mechanism of beta-cypermethrin resistance in Ae. albopictus, and to provide theoretical a basis for the rational use of insecticides according to the drug resistance of Ae. albopictus. Methods Beta-cypermethrin-resistant and -sensitive individuals of Ae. albopictus population in Changping were selected by biological assay. Total proteins were extracted for LC-MS/MS analysis. Differentially expressed proteins were identified by bioinformatics analysis and confirmed at the mRNA level. Results A total of 1 861 proteins were identified by bioinformatics analysis, of which 383 were differentially expressed, including 213 up-regulated and 170 down-regulated proteins in resistant individuals. Eight differentially expressed proteins related to drug metabolism according to KEGG pathway enrichment analysis (P<0.05) were selected for validation at the mRNA level. Alanine aminotransferase, UMP-CMP kinase, and glutathione S-transferase were up-regulated in resistant individuals. Conclusions There are differentially expressed proteins between beta-cypermethrin-resistant and -sensitive individuals of Ae. albopictus. Alanine aminotransferase, UMP-CMP kinase, and glutathione S-transferase are up-regulated in resistant individuals, which may be associated with beta-cypermethrin resistance.

Cite this article

HUA Dong-dong, WANG Lei, XIAO Di, LI Wen-yu, ZHOU Xin-xin, LUN Xin-chang, MU Qun-zheng, LIU Qi-yong, MA Wei, MENG Feng-xia . A comparative proteomics analysis of beta-cypermethrin resistance in Aedes albopictus population in Changping, Beijing, China[J]. Chinese Journal of Vector Biology and Control, 2023 , 34(2) : 196 -203 . DOI: 10.11853/j.issn.1003.8280.2023.02.009

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