Morphological and molecular identification of a non-recorded cockroach species, Diplopterina parva, inceperated at a port of China

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  • 1. State Key Laboratory of Medical Vector Surveillance, Zhuhai International Travel Healthcare Center, Zhuhai, Guangdong 519020, China;
    2. Health Quarantine Laboratory, Zhongshan Customs Technology Center, Zhongshan, Guangdong 528400, China;
    3. Zhongshan Torch Polytechnic, Zhongshan, Guangdong 528436, China;
    4. Qinhuangdao Customs, Qinhuangdao, Hebei 066002, China

Received date: 2022-05-24

  Online published: 2022-12-09

Supported by

General Administration of Customs Research Projects (No. 2020HK121, 2021HK144, 2021HK005);Zhongshan City Major Public Welfare Projects (No. 2021B2009); Gongbei Customs Research Projects (No. 2021GK009); Key R&D Program of Hebei Province (No. 213777109D)

Abstract

Objective To identify the species of two cockroaches intercepted in the import containers at Zhongshan port in China by morphological and molecular methods. Methods A female cockroach and a cockroach nymph were intercepted in the imported containers from Nigeria at Zhongshan port in November, 2021. The morphological characteristics of the female cockroach were retrieved by the morphological method, and the genomic DNA was extracted from the two cockroaches. The gene segment of mitochondrial cytochrome c oxidase subunit Ⅰ (COⅠ) was amplified by polymerase chain reaction and sequenced, and the sequencing results were compared in GenBank. The phylogenetic tree was constructed by neighbor-joining method. Results The female cockroach was morphologically identified as Diplopterina parva. The COⅠ gene sequences of the two cockroaches did not compare the homologous species with a similarity of more than 98% in GenBank, but the similarity of the COⅠ gene sequences of the two cockroaches was >98%. The cockroach nymph was identified as D. parva. It has been verified that no distribution of this species has been seen in China. Conclusion The two cockroaches intercepted in the imported containers at Zhongshan port are identified as D. parva by morphological and molecular methods, which is a non-recorded species in China.

Cite this article

LIU De-xing, LI Ting-ting, WEI Xiao-ya, YUE Qiao-yun, QIU De-yi, KE Ming-jian, NIE Wei-zhong, CHEN Jian . Morphological and molecular identification of a non-recorded cockroach species, Diplopterina parva, inceperated at a port of China[J]. Chinese Journal of Vector Biology and Control, 2022 , 33(6) : 859 -864 . DOI: 10.11853/j.issn.1003.8280.2022.06.018

References

[1] 刘峰,刘亚楠,孙钦同,等. 2015-2019年山东省综合超市蜚蠊侵害调查[J]. 预防医学论坛,2020,26(6):401-402,406. DOI:10.16406/j.pmt.issn.1672-9153.2020.06.001. Liu F,Liu YN,Sun QT,et al. Survey on cockroaches invasion in large supermarkets,Shandong province,2015-2019[J]. Prev Med Trib,2020,26(6):401-402,406. DOI:10.16406/j.pmt.issn.1672-9153.2020.06.001.(in Chinese)
[2] 凌浩宇,沈润华,郭升阳,等. 我国口岸输入性蜚蠊现状与应对措施[J]. 中国国境卫生检疫杂志,2021,44(2):142-144. DOI:10.16408/j.1004-9770.2021.02.019. Ling HY,Shen RH,Guo SY,et al. Status and countermeasures of imported cockroaches at ports[J]. Chin J Front Health Quar,2021,44(2):142-144. DOI:10.16408/j.1004-9770.2021.02.019.(in Chinese)
[3] 廖如燕,张显光,谢勇秋,等. 一艘入境巨轮上60余万只蜚蠊的卫生处理[J]. 中国国境卫生检疫杂志,2017,40(1):73-74. DOI:10.16408/j.1004-9770.2017.01.020. Liao RY,Zhang XG,Xie YQ,et al. Investigation and disposal on a case with more than 600 thousand cockroaches carried by an inbound giant vessel[J]. Chin J Front Health Quar,2017,40(1):73-74. DOI:10.16408/j.1004-9770.2017.01.020.(in Chinese)
[4] 魏晓雅,邱德义,岳巧云,等. 口岸截获国内未见分布蜚蠊:斯科特卡蠊的形态和DNA条形码鉴定[J]. 中国国境卫生检疫杂志,2016,39(6):381-384,449. DOI:10.16408/j.1004-9770.2016.06.001. Wei XY,Qiu DY,Yue QY,et al. Morphology and DNA barcoding identification of Cartoblatta scorteccii:A non-recorded cockroach species in China[J]. Chin J Front Health Quar,2016,39(6):381-384,449. DOI:10.16408/j.1004-9770.2016.06.001.(in Chinese)
[5] 魏晓雅,刘德星,邱德义,等. 中山口岸截获国内未见分布染色硬翅蠊的鉴定[J]. 中国媒介生物学及控制杂志,2018,29(1):23-26. DOI:10.11853/j.issn.1003.8280.2018.01.006. Wei XY,Liu DX,Qiu DY,et al. Identification of Ceratinoptera picta:A non-recorded cockroach species in China intercepted at Zhongshan port,China[J]. Chin J Vector Biol Control,2018,29(1):23-26. DOI:10.11853/j.issn.1003.8280.2018.01.006.(in Chinese)
[6] 刘宪伟,朱卫兵,戴莉,等. 中国东南部地区的蜚蠊[M]. 郑州:河南科学技术出版社,2017:19-40. Liu XW,Zhu WB,Dai L,et al. Cockroaches of southeastern China[M]. Zhengzhou:Henan Science and Technology Press,2017:19-40. (in Chinese)
[7] Shelford R. II. On some new species of Blattidae in the Oxford and Paris museums[J]. Ann Mag Nat Hist,1907,19(109):25-49. DOI:10.1080/00222930709487227.
[8] Borg H. Beiträge zur Kenntnis der insektenfauna von Kamerun. No. 10. Blattodeen aus Kamerun[J]. Bihang Till K Svenska Vet Akad Handlingar,1902,28(4):1-36.
[9] Roth LM. Systematics and phylogeny of cockroaches (Dictyoptera:Blattaria)[J]. Orient Insects,2003,37(1):1-186. DOI:10.1080/00305316.2003.10417344.
[10] Klass KD. The external male genitalia and the phylogeny of Blattaria and Mantodea[J]. Bonn Zool Monog,1997,42:1-341.
[11] Li XR,Zheng YH,Wang CC,et al. Old method not old-fashioned:Parallelism between wing venation and wing-pad tracheation of cockroaches and a revision of terminology[J]. Zoomorphology,2018,137(4):519-533. DOI:10.1007/s00435-018-0419-6.
[12] Kukalová-Peck J,Lawrence JF. Relationships among coleopteran suborders and major endoneopteran lineages:Evidence from hind wing characters[J]. Eur J Entomol,2004,101(1):95-144. DOI:10.14411/eje.2004.018.
[13] Folmer O,Black M,Hoeh W,et al. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates[J]. Mol Mar Biol Biotechnol,1994,3(5):294-299.
[14] Arslan A,Ilhak OI,Calicioglu M. Effect of method of cooking on identification of heat processed beef using polymerase chain reaction (PCR) technique[J]. Meat Sci,2006,72(2):326-330. DOI:10.1016/j.meatsci.2005.08.001.
[15] Princis K. La réserve naturelle intégrale du mont Nimba. Ⅳ. Dictyoptera,Blattariae[J]. Mém Inst franç Afr Noire,1963,66:119-162.
[16] Shelford R. New species of Blattidae in the collection of the Deutsche Entomologische National-Museum (Orthopt.)[J]. Dtsch Entomo Z,1908,115-131.
[17] 贺骥,聂维忠,陈帆,等. 国境口岸及输入性病媒生物控制面临的机遇与挑战[J]. 中华卫生杀虫药械,2017,23(5):480-483. DOI:10.19821/j.1671-2781.2017.05.023. He J,Nie WZ,Chen F,et al. The opportunities and challenges faced by frontier ports and imported vectors control[J]. Chin J Hyg Insec Equip,2017,23(5):480-483. DOI:10.19821/j.1671-2781.2017.05.023.(in Chinese)
[18] 胡学锋,陈梦茜,陶必林,等. 口岸输入性传染病防控措施的定性系统评价[J]. 中国国境卫生检疫杂志,2018,41(3):166-169. DOI:10.16408/j.1004-9770.2018.03.004. Hu XF,Chen MQ,Tao BL,et al. Qualitative systematic evaluation of prevention and control measures for imported infectious diseases at ports[J]. Chin J Front Health Quar,2018,41(3):166-169. DOI:10.16408/j.1004-9770.2018.03.004.(in Chinese)
[19] 邱德义,陈健,单振菊,等. 输入性病媒生物DNA条形码检疫鉴定新模式与发展前景[J]. 中国国境卫生检疫杂志,2017,40(1):67-71. DOI:10.16408/j.1004-9770.2017.01.018. Qiu DY,Chen J,Shan ZJ,et al. Discussion on the new model and its development prospects of the invasive medical vectors identification and quarantine[J]. Chin J Front Health Quar,2017,40(1):67-71. DOI:10.16408/j.1004-9770.2017.01.018.(in Chinese)
[20] 聂维忠,聂晨辉,贺骥,等. 开展21世纪海上丝绸之路沿线国家输入性病媒生物监测与防控研究,服务国家发展战略,切实维护国门安全[J]. 口岸卫生控制,2020,25(3):23-27. DOI:10.3969/j.issn.1008-5777.2020.03.007. Nie WZ,Nie CH,He J,et al. Developing the monitoring and control research on introduced vectors at countries along the 21st-century maritime silk road jointly serving the national development strategy and safeguarding the national gate security effectively[J]. Port Health Control,2020,25(3):23-27. DOI:10.3969/j.issn.1008-5777.2020.03.007.(in Chinese)
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