Objective To analyze cases'and host animals'specimens in a cluster outbreak of hemorrhagic fever with renal syndrome (HFRS) in an island industrial park in Daishan county,Zhejiang province,China in 2020,clarify the relationship between the cases and hosts and the molecular phylogenetic characteristics of the virus,and to provide a reference for local prevention and treatment of HFRS.Methods Serum samples from the cases and rodents were collected.Reverse transcription PCR was used to detect the viral nucleic acid.Nested PCR was used to amplify the M gene,followed by sequencing,genotyping,and homology analysis.Results From April to July 2020,a total of four confirmed cases of HFRS were reported in the island industrial park in Daishan county,and all were positive for anti-hantavirus IgG and IgM antibodies.Hantavirus RNA was present in the serum samples of two cases and four Rattus norvegicus rodents,all being Seoul virus (SEOV).The six samples'M gene fragments were 100% homologous,and were most closely related to the isolates Rod/2012/QHD/4/Gc from Hebei province and RuianRn180 from Ruian,Zhejiang.Conclusion This cluster outbreak was caused by the transmission of SEOV hantavirus through R.norvegicus.The virus strains exhibited high homology,obvious geographic clustering,and relative selectivity for hosts.
CHEN Xu-hui, TAN Qi-long, SHU Ji-wei, YE Ling, LI Shi-bo, WANG Qiu-jing
. Molecular epidemiological analysis of Hantavirus in an outbreak of hemorrhagic fever with renal syndrome in Daishan county,Zhejiang province,China[J]. Chinese Journal of Vector Biology and Control, 2022
, 33(5)
: 706
-709
.
DOI: 10.11853/j.issn.1003.8280.2022.05.017
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