Objective To investigate the resistance of Anopheles sinensis to commonly used insecticides and the mutation of knockdown resistance (kdr) related gene in the voltage-gated sodium channel (VGSC) and acetylcholinesterase gene 1(ace-1 gene) encoding acetylcholinesterase in Sanya,Hainan province,China.Methods An.sinensis adults were captured in Tianya district,Sanya,Hainan province from September to October 2018 and September 2020.The resistance of field-captured An.sinensis to five commonly used insecticides was tested.Survival samples were collected from resistance testing,which were inspected for the mutation at the 1014 locus in the VGSC gene and the 119 locus in the ace-1 gene by the direct sequencing method.Results In 2018 and 2020,An.sinensis showed a resistance to deltamethrin (0.05%) and dichlorodiphenyltrichloroethane (DDT)(4.00%),with the 24 h mortality rates of 43.00% and 11.11% in 2018,and 71.00% and 39.18% in 2020,respectively.The resistance to malathion (5.0%) was changed from 91.00%(possible resistance) in 2018 to 100%(susceptible) in 2020.Sequencing results of polymerase chain reaction products showed that one mutation was detected at the 1014 locus in the VGSC gene and the 119 locus in the ace-1 gene,respectively,i.e.,L1014F leucine (L) mutated to phenylalanine (F)(TTG mutated to TTT) and G119S glycine (G) mutated to serine (S)(GGC mutated to AGC),and the mutation frequencies were 48.75% and 57.14% in 2018 and 52.50% and 43.75% in 2020,respectively.Conclusion An.sinensis from Sanya is resistant to deltamethrin and DDT but susceptible to malathion,etofenprox,and chlorfenapyr.The resistance-conferring mutation may lead to the resistance of An.sinensis to insecticides in Sanya,Hainan province.
WU Qun, SUN Ding-wei, LI Shan-gan, ZENG Xue-xia, ZENG Lin-hai, WANG Shao-ping, WU De-lei, WEI Jia-jia, LI Ling-gang, CHEN Cheng-rong, LIU Ying, CHEN Yan
. Investigation on resistance and resistance-conferring mutations in Anopheles sinensis in Sanya,Hainan province,China[J]. Chinese Journal of Vector Biology and Control, 2022
, 33(5)
: 666
-671
.
DOI: 10.11853/j.issn.1003.8280.2022.05.010
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