Investigation on resistance and resistance-conferring mutations in <i>Anopheles sinensis</i> in Sanya,Hainan province,China

WU Qun, SUN Ding-wei, LI Shan-gan, ZENG Xue-xia, ZENG Lin-hai, WANG Shao-ping, WU De-lei, WEI Jia-jia, LI Ling-gang, CHEN Cheng-rong, LIU Ying, CHEN Yan

doi:10.11853/j.issn.1003.8280.2022.05.010

Chinese Journal of Vector Biology and Control >

2022 , Vol. 33 >Issue 5: 666 - 671

DOI: https://doi.org/10.11853/j.issn.1003.8280.2022.05.010

Experimental Study

Investigation on resistance and resistance-conferring mutations in Anopheles sinensis in Sanya,Hainan province,China

Expand

1. Department of Tropical and Chronic Disease Prevention and Control, Hainan Center for Disease Prevention and Control, Haikou, Hainan 570203, China;
2. Sanya Center for Disease Prevention and Control, Sanya, Hainan 572000, China;
3. Gaofeng Health Center of Tianya District of Sanya City, Sanya, Hainan 572022, China

Received date: 2022-03-29

Online published: 2022-10-14

Supported by

Hainan Province Science and Technology (No.ZDKJ2021035);Natural Science Foundation of Hainan Province of China (No.821MS0844,821QN420)

Fold

Abstract

Objective To investigate the resistance of Anopheles sinensis to commonly used insecticides and the mutation of knockdown resistance (kdr) related gene in the voltage-gated sodium channel (VGSC) and acetylcholinesterase gene 1(ace-1 gene) encoding acetylcholinesterase in Sanya,Hainan province,China.Methods An.sinensis adults were captured in Tianya district,Sanya,Hainan province from September to October 2018 and September 2020.The resistance of field-captured An.sinensis to five commonly used insecticides was tested.Survival samples were collected from resistance testing,which were inspected for the mutation at the 1014 locus in the VGSC gene and the 119 locus in the ace-1 gene by the direct sequencing method.Results In 2018 and 2020,An.sinensis showed a resistance to deltamethrin (0.05%) and dichlorodiphenyltrichloroethane (DDT)(4.00%),with the 24 h mortality rates of 43.00% and 11.11% in 2018,and 71.00% and 39.18% in 2020,respectively.The resistance to malathion (5.0%) was changed from 91.00%(possible resistance) in 2018 to 100%(susceptible) in 2020.Sequencing results of polymerase chain reaction products showed that one mutation was detected at the 1014 locus in the VGSC gene and the 119 locus in the ace-1 gene,respectively,i.e.,L1014F leucine (L) mutated to phenylalanine (F)(TTG mutated to TTT) and G119S glycine (G) mutated to serine (S)(GGC mutated to AGC),and the mutation frequencies were 48.75% and 57.14% in 2018 and 52.50% and 43.75% in 2020,respectively.Conclusion An.sinensis from Sanya is resistant to deltamethrin and DDT but susceptible to malathion,etofenprox,and chlorfenapyr.The resistance-conferring mutation may lead to the resistance of An.sinensis to insecticides in Sanya,Hainan province.

Key words： Anopheles sinensis; Insecticides; Voltage-gated sodium channel gene; Acetylcholinesterase gene 1

Cite this article

WU Qun, SUN Ding-wei, LI Shan-gan, ZENG Xue-xia, ZENG Lin-hai, WANG Shao-ping, WU De-lei, WEI Jia-jia, LI Ling-gang, CHEN Cheng-rong, LIU Ying, CHEN Yan . Investigation on resistance and resistance-conferring mutations in Anopheles sinensis in Sanya,Hainan province,China[J]. Chinese Journal of Vector Biology and Control, 2022 , 33(5) : 666 -671 . DOI: 10.11853/j.issn.1003.8280.2022.05.010

References

[1] World Health Organization. World malaria report 2020[R]. Geneva:WHO,2020.
[2] 中华人民共和国国家卫生健康委员会疾病预防控制局. 2020年全国法定传染病疫情概况[EB/OL].(2021-03-12)[2022-04-17]. http://www.nhc.gov.cn/jkj/s3578/202103/f1a448b7df7d4760976fea6d55834966.shtml. Bureau of Disease Prevention and Control,Nationl Health Commission of the People's Republic of China. Overview of the national epidemic of notifiable infectious diseases in 2020[EB/OL].(2021-03-12)[2022-04-17]. http://www.nhc.gov.cn/jkj/s3578/202103/f1a448b7 df7 d4760976fea6 d55834966.shtml.(in Chinese)
[3] 董昊炜,南春燕,周秋明,等.基于16S rRNA高通量测序的现场中华按蚊及其孳生地水体菌群结构研究[J].中国媒介生物学及控制杂志,2021,32(5):533-540. DOI:10.11853/j.issn.1003.8280.2021.05.005. Dong HW,Nan CY,Zhou QM,et al. Composition of microbiota in Anopheles sinensis and water in larvae breeding site:A study based on 16S rRNA high-throughput sequencing[J]. Chin J Vector Biol Control,2021,32(5):533-540. DOI:10.11853/j.issn.1003.8280.2021.05.005.(in Chinese)
[4] 曹俊,刘耀宝,曹园园,等.中国消除疟疾的持续挑战:输入性疟疾[J].中国寄生虫学与寄生虫病杂志,2018,36(2):93-96. Cao J,Liu YB,Cao YY,et al. Sustained challenge to malaria elimination in China:Imported malaria[J]. Chin J Parasitol Parasit Dis,2018,36(2):93-96.(in Chinese)
[5] 赵宣,侯乃旭,陈晨,等.海南省传播病毒蚊媒种类及蚊媒病毒流行情况分析[J].海南医学,2017,28(7):1174-1179. DOI:10.3969/j.issn.1003-6350.2017.07.052. Zhao X,Hou NX,Chen C,et al. Analysis of mosquito vector species and epidemic situation of mosquito-borne viruses in Hainan province[J]. Hainan Med J,2017,28(7):1174-1179. DOI:10.3969/j.issn.1003-6350.2017.07.052.(in Chinese)
[6] Ni RY,Liu N,Li M,et al. Identification and phylogenetic analysis of voltage-gated sodium channel haplotypes in the malaria vector Anopheles sinensis using a high-throughput amplicon sequencing approach[J]. Parasit Vectors,2021,14(1):499. DOI:10.1186/s13071-021-05009-5.
[7] 林康明,杨益超,黎军.我国主要传疟媒介抗药性研究进展[J].中国热带医学,2019,19(6):584-590. DOI:10.13604/j.cnki.46-1064/r.2019.06.21. Lin KM,Yang YC,Li J. Progress of insecticide resistance of the main malaria vectors in China[J]. China Trop Med,2019,19(6):584-590. DOI:10.13604/j.cnki.46-1064/r.2019.06.21(in Chinese)
[8] 胡亚博,贺志权,刘颖,等.河南省濮阳市中华按蚊对杀虫剂敏感性调查[J].中国血吸虫病防治杂志,2021,33(5):501-504,509. DOI:10.16250/j.32.1374.2020331. Hu YB,He ZQ,Liu Y,et al. Susceptibility of Anopheles sinensis to insecticides in Puyang city,Henan province[J]. Chin J Schisto Control,2021,33(5):501-504,509. DOI:10.16250/j.32.1374. 2020331.(in Chinese)
[9] 王纯玉,李志,丁俊,等.辽宁省东港市蚊虫分布及中华按蚊抗药性调查[J].中华卫生杀虫药械,2021,27(3):223-225. DOI:10.19821/j.1671-2781.2021.03.009. Wang CY,Li Z,Ding J,et al. Distribution of mosquitoes and insecticide resistance of Anopheles sinensis in Donggang city of Liaoning province[J]. Chin J Hyg Insect Equip,2021,27(3):223-225. DOI:10.19821/j.1671-2781.2021.03.009.(in Chinese)
[10] 王善青.海南疟疾70年:从肆虐走向消除[J].中国热带医学,2019,19(8):707-718. DOI:10.13604/j.cnki.46-1064/r.2019. 08.01. Wang SQ. The seventy years of malaria from hyperendemicity to elimination in Hainan[J]. China Trop Med,2019,19(8):707-718. DOI:10.13604/j.cnki.46-1064/r.2019.08.01.(in Chinese)
[11] 林常勇,王善青,陈朱,等.海南省三亚市1名间日疟原虫带虫者的调查与处置[J].中国热带医学,2016,16(12):1243-1245. DOI:10.13604/j.cnki.46-1064/r.2016.26. Lin CY,Wang SQ,Chen Z,et al. Investigation and deposition of a Plasmodium vivax carrier in Sanya city,Hainan province[J]. China Trop Med,2016,16(12):1243-1245. DOI:10.13604/j.cnki.46-1064/r.2016.26.(in Chinese)
[12] 林常勇,陈朱,王善青,等.海南省三亚市一起罕见的当地三日疟疫情分析及处置[J].中国热带医学,2016,16(5):481-484. DOI:10.13604/j.cnki.46-1064/r.2016.05.20. Lin CY,Chen Z,Wang SQ,et al. Investigation of a rare local epidemic of Plasmodium malariae infection in Sanya city,Hainan province[J]. China Trop Med,2016,16(5):481-484. DOI:10.13604/j.cnki.46-1064/r.2016.05.20.(in Chinese)
[13] 孙定炜,王善青,卓开仁,等.海南省中华按蚊对3种杀虫剂的抗药性研究[J].中国寄生虫学与寄生虫病杂志,2014,32(2):127-129. Sun DW,Wang SQ,Zhuo KR,et al. Resistance of Anopheles sinensis to three common insecticides in Hainan province[J]. Chin J Parasitol Parasit Dis,2014,32(2):127-129.(in Chinese)
[14] Sun DW,Wang GZ,Zeng LH,et al. Extensive resistance of Anopheles sinensis to insecticides in malaria-endemic areas of Hainan province,China[J]. Am J Trop Med Hyg,2017,97(1):295-298. DOI:10.4269/ajtmh.16-0723.
[15] Qin Q,Li YJ,Zhong DB,et al. Insecticide resistance of Anopheles sinensis and An. vagus in Hainan Island,a malaria-endemic area of China[J]. Parasit Vectors,2014,7:92. DOI:10.1186/1756-3305-7-92.
[16] 陆宝麟.中国动物志.昆虫纲.第九卷.双翅目:蚊科(下卷)[M].北京:科学出版社,1997:16-17. Lu BL. Fauna Sinica. Insecta,Vol 9. Diptera:Culicidae 2[M]. Beijing:Science Press,1997:16-17.(in Chinese)
[17] 中华人民共和国卫生部. GB/T 26347-2010蚊虫抗药性检测方法生物测定法[S].北京:中国标准出版社,2011. Ministry of Health of the People's Republic of China. GB/T 26347-2010 est methods of mosquito resistance to insecticides-Bioassay methods[S]. Beijing:Standards Press of China,2011.(in Chinese)
[18] 梁秋果.贵州省中华按蚊抗药性靶标kdr、ace-1基因突变检测及种群遗传学研究[D].贵阳:贵州医科大学,2019. Liang QG. Detection of mutations in kdr and ace-1 genes of resistance targets and population genetics of Anopheles sinensis in Guizhou province,China[D]. Guiyang:Guizhou Medcial University,2019.(in Chinese)
[19] Yang C,Feng XY,Huang ZS,et al. Diversity and frequency of kdr mutations within Anopheles sinensis populations from Guangxi,China[J]. Malar J,2016,15(1):411. DOI:10.1186/s12936-016-1467-3.
[20] Chang XL,Zhong DB,Fang Q,et al. Multiple resistances and complex mechanisms of Anopheles sinensis mosquito:A major obstacle to mosquito-borne diseases control and elimination in China[J]. PLoS Negl Trop Dis,20148(5):e2889. DOI:10.1371/journal.pntd.0002889.
[21] Busvine JR. Mechanism of resistance to insecticide in houseflies[J]. Nature,1951,168(4266):193-195. DOI:10.1038/168193a0.
[22] Silva APB,Santos JMM,Martins AJ. Mutations in the voltage-gated sodium channel gene of anophelines and their association with resistance to pyrethroids:A review[J]. Parasit Vectors,2014,7:450. DOI:10.1186/1756-3305-7-450.
[23] Zhong DB,Chang XL,Zhou GF,et al. Relationship between knockdown resistance,metabolic detoxification and organismal resistance to pyrethroids in Anopheles sinensis[J]. PLoS One,2013,8(2):e55475. DOI:10.1371/journal.pone.0055475.
[24] Baek JH,Kim HW,Lee WJ,et al. Frequency detection of organophosphate resistance allele in Anopheles sinensis(Diptera:Culicidae) populations by real-time PCR amplification of specific allele (rtPASA)[J]. J Asia-Pac Entomol,2006,9(4):375-380. DOI:10.1016/S1226-8615(08)60317-9.
[25] Yang C,Feng XY,Liu N,et al. Target-site mutations (AChE-G119S and kdr) in Guangxi Anopheles sinensis populations along the China-Vietnam border[J]. Parasit Vectors,2019,12(1):77. DOI:10.1186/s13071-019-3298-x.
[26] Qian WP,Liu N,Yang Y,et al. A survey of insecticide resistance-conferring mutations in multiple targets in Anopheles sinensis populations across Sichuan,China[J]. Parasit Vectors,2021,14(1):169. DOI:10.1186/s13071-021-04662-0.
[27] Fang Y,Shi WQ,Wu JT,et al. Resistance to pyrethroid and organophosphate insecticides,and the geographical distribution and polymorphisms of target-site mutations in voltage-gated sodium channel and acetylcholinesterase 1 genes in Anopheles sinensis populations in Shanghai,China[J]. Parasit Vectors,2019,12(1):396. DOI:10.1186/s13071-019-3657-7.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References