Objective To investigate the species of ticks and tick-borne viruses in Huanan county,Jiamusi city,Heilongjiang province,China.Methods Ticks were collected in Huanan in May 2020 and identified according to morphological keys.Real-time PCR was used to detect the RNA of viruses in the collected ticks,i.e.,tick-borne encephalitis virus (TBEV),severe fever with thrombocytopenia syndrome virus (SFTSV),Omsk hemorrhagic fever virus (OHFV),Langat virus (LGTV),Powassan virus (POWV),and Alongshan virus (ALSV).High-throughput sequencing was used to obtain the genome sequences of detected viruses.Results A total of 890 ticks were collected,including 475 Ixodes persulcatus(210 males and 265 females),360 Dermacentor silvarum(260 males and 100 females),and 55 Haemaphysalis concinna(5 males and 50 females).One batch of tick specimens (n=10;JMS20) was positive for TBEV,which was carried by female I. persulcatus.Other viruses were not detectable.Phylogenetic analysis showed that this TBEV strain was closely related to the Far Eastern subtype of TBEV.Conclusion Ticks in Jiamusi city still carry TBEV,and our results provide basic data for the prevention and control of tick-borne virus diseases in the region.
LI Fan, LIU Feng-ming, FENG Pan-pan, WANG Dong-qiang, FU Shi-hong, NIE Kai, YIN Qi-kai, HE Ying, YIN Jia-yu, XU Song-tao, BAO Ming-jia, WANG Huan-yu
. Investigation of tick-borne viruses in Huanan county,Heilongjiang province,China,2020[J]. Chinese Journal of Vector Biology and Control, 2022
, 33(5)
: 637
-641
.
DOI: 10.11853/j.issn.1003.8280.2022.05.005
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