Objective To develop the method for ticks to feed on mouse blood under laboratory conditions.Methods The blood feeding chamber for ticks was prepared with commercially available materials.The attachment,engorgement,growth,and reproduction of Haemaphysalis longicornis were evaluated after feeding on mouse blood.Results The chambers were used to feed H.longicornis.Nymphs used 1-4 d to attach themselves to mice and had a mean biting success rate of 80.00%,an engorgement duration of 4-6 d and a mean engorgement rate of 98.21%.On the average,97.85% nymphs molted to adults.Adult ticks spent 6.5 d (M) to engorge with an engorgement rate of 66.67%,and the body weight of engorged ticks increased>150-fold versus unfed ticks.Each female tick laid an average of 876 eggs.On the average,78.90% eggs hatched with the duration of 25 d (M).Conclusion The blood feeding chamber for ticks developed by the authors is suitable for rearing H.longicornis under laboratory conditions.
ZHENG Wei-qing, FU Ren-long, TAO Hui-ying, LIU Yang-qing, CHEN Sheng-en
. Laboratory method and evaluation of Haemaphysalis longicornis feeding on mouse blood[J]. Chinese Journal of Vector Biology and Control, 2022
, 33(5)
: 627
-631
.
DOI: 10.11853/j.issn.1003.8280.2022.05.003
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