Vector-borne Disease

Detection of Rickettsia raoultii in Rhipicephalus sanguineus parasitized on the body surfaces of dogs in pet centers in Wudang district,Guizhou,China

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  • 1. School of Public Health and Health, Guizhou Medical University, Guiyang, Guizhou 550025, China;
    2. Central Laboratory, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550002, China;
    3. Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550001, China;
    4. Academy of Military Medicine, Academy of Military Sciences, Beijing 100001, China

Received date: 2022-02-20

  Online published: 2022-08-12

Supported by

National Natural Science Foundation of China (No. 82160633,81760605)

Abstract

Objective To investigate the Rickettsia infection and species of pet-dog-parasitizing ticks by the molecular biological technique. Methods Tick samples were collected from the body surfaces of pet dogs in pet centers in Wudang district, Guiyang, China from March to May 2021. Tick species identification and the polymerase chain reaction detection and genotyping of citrate synthase gene (gltA) and outer membrane protein A gene (ompA) of tick-borne Rickettsia were performed. Results A total of 133 ticks were collected from the body surfaces of pet dogs. The morphological characteristics and tick 16S rRNA amplification confirmed that all collected ticks were Rhipicephalus sanguineus. Rickettsia-specific ompA gene amplification, sequencing, and phylogenetic analysis confirmed that R. sanguineus were infected with R. raoultii. Conclusion R. sanguineus is found to parasitize the body surfaces of pet dogs and be infected with R. raoultii, indicating a risk of Rickettsia infecting humans through pet-dog-parasitizing ticks. It is recommended for the local health authorities to make further assessment of the pathogenicity and public health risks of pet-dog-parasitized tick-borne Rickettsia.

Cite this article

MENG Jiao, CHEN Xing-xing, ZHANG Xia, YU Fu-xun, WU Jia-hong, YANG Guang-hong, JIANG Jia-fu, SUN Yi, CAO Wu-chun, ZHAN Lin . Detection of Rickettsia raoultii in Rhipicephalus sanguineus parasitized on the body surfaces of dogs in pet centers in Wudang district,Guizhou,China[J]. Chinese Journal of Vector Biology and Control, 2022 , 33(4) : 536 -542 . DOI: 10.11853/j.issn.1003.8280.2022.04.018

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