Objective To screen and purify stable strains highly resistant to beta-cypermethrin using the field samples of Musca domestica in Hangzhou, China. Methods Houseflies were collected from different locations in Hangzhou, and adult houseflies were screened by direct spray with beta-cypermethrin. After screening for 5 generations, the houseflies were maintained for two years. Resistance was determined using topical application method for F2 against deltamethrin, beta-cypermethrin, dichlorvos, triclosan, and propoxur, for F7 against deltamethrin and beta-cypermethrin, and for houseflies maintained for 1 and 2 years against deltamethrin and beta-cypermethrin. The resistance data of sensitive strains were provided by the department of infectious diseases, Zhejiang provincial center for disease control and prevention. Results The F2 generation of M. domestica in Hangzhou developed resistance to the five commonly used insecticides. The LD50of propoxur was >400.000 0 μg/♀, with the highest resistance and R/S >1 345.00. Resistance to other insecticide showed R/S between 6.79 and 77.33. The R/Sfor deltamethrin was 77.33 (LD50=0.069 6μg/♀) and the R/S for beta-cypermethrin was 55.58 (LD50=0.200 1 μg/♀). After being screened with beta-cypermethrin for 5 consecutive generations, compared with F2, F7 showed 29.89% and 51.27% increases in resistance to deltamethrin and beta-cypermethrin, respectively. After 2 years of normal rearing, the beta-cypermethrin-resistant strain showed decreased resistance as compared with the F7 generation; however, compared with sensitive strains, the R/S values for deltamethrin and beta-cypermethrin were 70.22 and 56.25, respectively, and the resistance remained at high levels. The LD50 for propoxur remained above 400.000 0 μg/♀. Conclusion After being screened with beta-cypermethrin for 5 generations and maintaining for two years, relatively stable beta-cypermethrin-resistant strains of M. domestica in Hangzhou were obtained, which lays a foundation for further research on the resistance mechanism of M. domestica.
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