Objective To investigate the role of blood-eating insects in the family Tabanidae in the spread of African swine fever virus (ASFV) in the Dabie Mountain region of Jinzhai county, Anhui province, China, and to provide evidence for the prevention and control of Africian swine fever in the region. Methods The insect trap method was used to collect wild horsefly in the Dabie Mountain region of Jinzhai county, Anhui province in September 2019. The collected horseflies were observed with a stereo microscope to preliminarily identify their species based on their morphological characteristics. The collected horseflies were separately ground and DNA was extracted using a tissue nucleic acid extraction kit and a virus nucleic acid extraction kit. Cytochrome c oxidase subunit Ⅰ (COⅠ) gene amplification and sequencing were performed on the tissue genome to identify the species of the horseflies. With the ASFV p72 gene primers published by World Organisation for Animal Health, the ASFV nucleic acid was amplified by PCR, followed by sequencing to detect the DNA of ASFV in the horsefly samples. Results A total of 24 horseflies were collected from three different locations. Through morphological observations and COⅠgene amplification and sequencing, the horseflies were identified as Tabanus hypomacros. Amplification with the ASFV p72 gene primers was positive for part of viral nucleic acid extraction samples, with an overall positive detection rate of ASFV of 45.83%. The product of the positive amplification with ASFV p72 primers was sequenced to be a 278 nt sequence. The phylogenetic analysis showed that the ASFV in the horsefly specimens and the epidemic ASFV strains in China were in the same evolutionary branch. Conclusion ASFV is found in wild horseflies in the Dabie Mountain region of Jinzhai county, Anhui province, and is highly homologous to the epidemic ASFV strains in China, indicating that T. hypomacros can be a potential mechanical transmission vector of ASFV.
ZHANG Xiang, CHENG Rong-rong, LIU Lu-yao, SUN Meng, HONG Yu-hang, YANG Cong-shan, LIU Yan, YIN Zong-jun, XU Qian-ming
. Tabanus hypomacros: A suspected mechanical transmission vector of African swine fever virus in Dabie Mountain region of Jinzhai county, Anhui province in China[J]. Chinese Journal of Vector Biology and Control, 2022
, 33(3)
: 326
-330
.
DOI: 10.11853/j.issn.1003.8280.2022.03.002
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