Objective To investigate the distribution of ticks and tick-borne pathogens in the central and western ports areas of the Inner Mongolia Autonomous Region (Inner Mongolia), China. Methods From April to July 2020, ticks at Ceke, Ganqimaodu, Mandula and Zhuengadabuqi ports of Inner Mongolia were collected and identified. Reverse transcription polymerase chain reaction (RT-PCR) and PCR were used to detect the Coxiella burnetii, Ehrlichia, spotted fever group Rickettsiae, Borrelia burgdorferi, tick-borne encephalitis virus, Anaplasma phagocytophilum, and severe fever with thrombocytopenia syndrome bunyavirus (SFTSV) in tick samples. The Chi-square test was used to analyze tick-borne pathogens in different regions. Results A total of 586 parasitic ticks from four species were identified. The dominant tick species were Dermacentor nuttalli at Mandula port (93.62%) and Zhuengadabuqi port (100%), and Hyalomma asiaticum at Ceke port (99.17%) and Ganqimaodu port (100%). The PCR results showed that C. burnetii was carried in 118 ticks, spotted fever group Rickettsiae in 207 ticks, A. phagocytophilum in 21 ticks, Ehrlichia in 16 ticks, SFTSV in 1 tick, and multiple pathogens in 80 ticks. Conclusion The central and western ports of Inner Mongolia are located in the border regions between China and Mongolia, where the tick-borne pathogens are complex. It is necessary to further strengthen tick-borne disease surveillance and prevention in this region.
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