Objective To study the factors affecting the sensitivity of adult Culex pipiens pallens to pyrethroid insecticides, and to provide evidence for the insecticide resistance surveillance and the control of adult Cx. pipiens pallens. Methods The WHO tube test was performed on laboratory Cx. pipiens pallens strains (the Beijing[BJ] strain, Beijing sensitive[BJS] strain, and Tianjin[TJ] strain) to evaluate the insecticide susceptibility of adult test mosquitoes to permethrin and deltamethrin under different conditions. Results In female adult mosquitoes, the sensitivity to permethrin was the BJ strain ≈ the BJS strain > the TJ strain (≈ meaning no significant difference in sensitivity between the two strains), and the median lethal concentrations (LC50) were 0.258% (0.222%, 0.304%), 0.283% (0.239%, 0.334%), and 0.457% (0.413%, 0.508%), respectively. In male adult mosquitoes, the sensitivity to permethrin was the BJ strain > the BJS strain ≈ the TJ strain, and the LC50 values were 0.174% (0.157%, 0.195%), 0.249% (0.213%, 0.290%), and 0.251% (0.205%, 0.294%), respectively. The sensitivity of female adult mosquitoes to deltamethrin was the BJ strain > the BJS strain ≈ the TJ strain, and the LC50 values were 0.024% (0.021%, 0.028%), 0.080% (0.066%, 0.099%), and 0.098% (0.079%, 0.127%), respectively. The sensitivity of male adult mosquitoes to deltamethrin was the BJ strain ≈ the BJS strain, and the LC50 values were 0.038% (0.032%, 0.449%) and 0.018% (0.011%, 0.034%), respectively. The knockdown resistance mutation rate was 15.96% in the BJ strain, while no mutation of the gene was detected in the BJS strain and TJ strain. Under laboratory conditions, the natural mortality of male adult Cx. pipiens pallens was higher than that of female mosquitoes at any age. Under the same conditions, male adult mosquitoes were more sensitive to the insecticides than female adult mosquitoes. The toxicity of deltamethrin was 3.5-10.8 times that of permethrin. Conclusion Age and sex can affect the insecticide susceptibility of adult mosquito populations. Therefore, when carrying out insecticide resistance monitoring or efficacy evaluation, test mosquitoes should be homogeneous.
LI Wen-yu, ZHOU Xin-xin, LUN Xin-chang, MA De-long, LIN Ling-hong, SONG Xiu-ping, LU Liang, LIU Qi-yong, WANG Jun, WANG Chun-lan, MENG Feng-xia
. A study of factors affecting the sensitivity of adult Culex pipiens pallens to pyrethroid insecticides[J]. Chinese Journal of Vector Biology and Control, 2022
, 33(2)
: 171
-176
.
DOI: 10.11853/j.issn.1003.8280.2022.02.002
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