Objective To identify the adult flies and pupae captured in the entry goods by Qingdao Airport Customs using morphological and DNA barcoding techniques. Methods Morphological identification was performed for the flies captured, and one leg of the fly and the pupae were used to extract genomic DNA. The DNA barcoding technique was used for the amplification and sequencing of the genomic DNA of this unknown fly species, and the sequences obtained were compared with those in GenBank database of National Center for Biotechnology Information and Barcode of Life Data Systems (BOLD) to construct an evolutionary tree. Results Morphological identification showed that the flies were identified as Phoridae in Diptera. DNA barcoding identification and sequence alignment showed that the DNA barcoding sequences of the adult fly and pupae were >99.00% homologous with the sequence of Megaselia spiracularis in BOLD and GenBank, and with reference to morphological identification, the species was identified as M. spiracularis. Conclusion The adult fly and pupae captured are identified as M. spiracularis, which is found in the entry goods by Qingdao Airport Customs for the first time.
XU Ying, LIU Hai-jiang, PANG Ying-jie, CHEN Xiao-guang, ZHANG Juan, ZHANG Jin, XU He-fei, TENG Xin-dong
. Imported Megaselia spiracularis captured in the entry goods at Qingdao port of China[J]. Chinese Journal of Vector Biology and Control, 2022
, 33(1)
: 133
-136
.
DOI: 10.11853/j.issn.1003.8280.2022.01.024
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