Objective To investigate the resistance of larval and adult Culex pipiens pallens to commonly used insecticides in the Dongping Lake area of Shandong province, China, and to provide evidence for rational use of insecticides for mosquito control. Methods The field populations of Cx. pipiens pallens were collected from the Dongping Lake area on July 7, 2021, and were reared to F1 generation in the laboratory. According to the bioassay methods of World Health Organization, the impregnation method was used for larval tests, and the median lethal dose and regression equation were calculated; the cylinder bioassay was used to determine the resistance of adult mosquitoes. The results were recorded with Excel 2019 software for statistical analysis. Results The resistance ratio of Cx. pipiens pallens larvae in the Dongping Lake area was 8.55 to DDVP (low resistance), 6.52 to propoxur (low resistance), 180.00 to deltamethrin (high resistance), 100.50 to cypermethrin (high resistance), and 1.50 to Bacillus thuringiensis subsp. israelensis (susceptible). For adult Cx. pipiens pallens, the mortality rate within 24 h after 1-h insecticide exposure was 16.00% for 0.025% deltamethrin (high resistance), 20.00% for 0.025% beta-cypermethrin (high resistance), 100% for 5% malathion (susceptible), and 88.00% for 0.01% propoxur (possible resistance). Conclusion Cx. pipiens pallens mosquitoes in the Dongping Lake area of Shandong province have developed varying degrees of resistance to the commonly used insecticides. It is necessary to strengthen the monitoring of mosquito resistance to insecticides in this region and take rational mosquito control measures to mitigate the development of mosquito resistance to insecticides and improve the effect of prevention and control.
LYU Wen-xiang, CHENG Peng, PENG Hui, WANG Hai-yang, WANG Hai-fang, GUO Xiu-xia, ZHANG Chong-xing, LIU Hong-mei, GONG Mao-qing, LIU Li-juan
. Insecticide resistance of Culex pipiens pallens in Dongping Lake area， Shandong province， China，in 2021[J]. Chinese Journal of Vector Biology and Control, 2022
, 33(1)
: 104
-107
.
DOI: 10.11853/j.issn.1003.8280.2022.01.019
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