Experimental Study

Transcriptome analysis of Cimex hemipterus (Hemiptera: Cimicidae)

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  • 1. Laboratory of Invasion Biology, School of Agricultural Sciences, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China;
    2. Department of Entomology, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA

Received date: 2021-08-16

  Online published: 2022-02-17

Supported by

Jiangxi Province 2018"Double Thousand Talents"Plan (No. jxsq2018102116); Jiangxi Province National Foreign Experts Program (No. G20200222010, G2021022002)

Abstract

Objective To obtain the transcriptome data of Cimex hemipterus and to provide a basis for subsequent functional genomic studies. Methods The mixed sample of C. hemipterus was sequenced on the Illumina NovaSeq 6000 high-throughput sequencing platform. Unigenes were obtained by assembling the sequencing data using the Trinity software for alignment and functional annotation against the databases of non-redundant protein sequence (NR), Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Evolutionary Genealogy of Genes: Non-supervised Orthologous Groups (eggNOG), and Swiss-Prot. The unigenes were analyzed for simple sequence repeat (SSR) loci with MISA and single nucleotide polymorphism (SNP) loci with Samtools and GATK softwares. Results A total of 5.49 Gb high-quality data were obtained from quality-controlled raw sequencing data. A total of 21 619 unigenes with a total length of 29 322 540 bp and an average length of 1 356 bp were obtained. Alignment and functional annotation of the unigenes sequences with five public databases resulted in 45 362 functional unigenes, 10 906 coding sequences (CDSs), 7 754 SSR loci, and 33 144 SNPs. Conclusion The transcriptome database of C. hemipterus was established and 21 619 unigenes were assembled for functional annotation analysis, which lays a foundation for subsequent studies of C. hemipterus in terms of functional genes, molecular marker development of SSR and SNP, genetic diversity, and genetic maps.

Cite this article

LI Ting, LIAO Song, XU Ye, WANG Chang-lu, WANG Jian-guo . Transcriptome analysis of Cimex hemipterus (Hemiptera: Cimicidae)[J]. Chinese Journal of Vector Biology and Control, 2022 , 33(1) : 54 -61 . DOI: 10.11853/j.issn.1003.8280.2022.01.010

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